Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the correspondin...

the aim of this work was to study the antimicrobial activity of tomato skin lycopene oleoresin against pseudomonas aeruginosa، escherichia coli، staphylococcus ureuse، salmonella typh ، l. monocytogenes ، bacillus cereus، bacillus licheniformis. oleoresin was extracted from tomato peel. lycopene content was measured by spectrophotometer. lycopene oleoresin contained 2321mg lycopene/ 100 g oleor...

A detailed characterization was performed of the Whipple's disease bacillus, strain Twist-MarseilleT, isolated from the cardiac valve of a patient with Whipple's disease bacillus endocarditis. This strain was isolated and maintained on human embryonic lung fibroblast monolayers, but could not be cultivated in the absence of living eukaryotic cells. Two morphological forms were observed, with di...

Previous studies have suggested that heterotrimeric G(i) proteins, Src tyrosine kinase and phosphatidylinositol-3 kinase (PI3 Kinase) are involved in signaling events induced by lipopolysaccharide (LPS) leading to pro-inflammatory cytokines gene expression. To investigate the involvement of these mediators in Gram-positive bacteria induced pro-inflammatory cytokine expression, LPS (10 ng/ml), h...

The objective of this study was to investigate how the relationships between bacterial communities and organic C (SOC) in topsoil (0-5 cm) are affected by tillage practices [conventional intensive tillage (CT) or no-tillage (NT)] and straw-returning methods [crop straw returning (S) or removal (NS)] under a rice-wheat rotation in central China. Soil bacterial communities were determined by high...

The presence of shared conserved insertion or deletions (indels) in protein sequences is a special type of signature sequence that shows considerable promise for phylogenetic inference. An alternative model of microbial evolution based on the use of indels of conserved proteins and the morphological features of prokaryotic organisms is proposed. In this model, extant archaebacteria and gram-pos...

This study was undertaken to investigate the effects of Bonny light crude oil (specific gravity = 0.81; API gravity 43.2 0 ) on the numerical composition of soil bacteria and fungi and relative distribution of Gram positive and Gram negative soil bacteria. Eight different levels of the crude oil (0.5, 1.0, 2.0, 2.5, 5.0, 10.0, 15.0 or 20.0% v/w of soil) were used for the controlled pollution of...

B acterial cytoplasmic membranes consist primarily of amphipathic phospholipids with lesser amounts of glycolipids, providing a degree of protection from deleterious, toxic substances that is lacking in organisms having a single membrane. Almost all bacterial membranes are assembled in bilayers with embedded integral and associated peripheral membrane proteins. In the case of gram-negative bact...