Shi‘ite Islam posits that medical technologies such as cloning or genetic engineering are not a change in God’s creation and cannot be forbidden for this reason. The study presents the main religious­-legal arguments opinion difference between view of theology jurisprudence interpretation term “change creation”. Jurisprudence emphasizes spiritual nature (deviation from...

objectives: in present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. materials and methods: we have cloned bovine prochymosin gene in methylotrophic yeast, p. pastoris, using ppic9k as an expression vector. the recombinant plasmid was transformed into the host by electroporation, and it was expressed in optimum con­ditions...

background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...

conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

The impossibility of perfectly copying or cloning an unknown quantum state is one of the basic rules governing the physics of quantum systems. The processes that perform the optimal approximate cloning have been found in many cases. These “quantum cloning machines” are important tools for studying a wide variety of tasks, e.g., state estimation and eavesdropping on quantum cryptography. This pa...

Background Cellulose is a homopolysaccharide composed of extracellular D-glucose monomers connected by glycosidic linkages in b-1,4 conformation which can be synthesized by a variety of living organisms, possessing numerous applications in food, pharmaceutical, medical area, etc.[1][2]. In 1990, cellulose synthase operon encoding four proteins required for bacterial cellulose (BC) biosynthesis ...

Typical reverse genetics systems for generating influenza viruses require the insertion of each genome segments by DNA ligation into vectors for genome synthesis and expression. Herein is described the construction and use of a novel pair of plasmid vectors for cloning all eight genome segments of influenza A virus by homologous recombination for influenza virus reverse genetics. Plasmids, pLLB...

GABA(B) receptors are broadly expressed in the nervous system and have been implicated in a wide variety of neurological and psychiatric disorders. The cloning of the first GABA(B) receptor cDNAs in 1997 revived interest in these receptors and their potential as therapeutic targets. With the availability of molecular tools, rapid progress was made in our understanding of the GABA(B) system. Thi...

Code duplication, or code cloning, is a common phenomena in the development of large software systems. Developers have a love-hate relationship with cloning. On one hand, cloning speeds up the development process. On the other hand, clone management is a challenging task as software evolves. Cloning has commonly been considered as undesirable for software maintenance and several research effort...