The objectives of the present study were isolation, identification and characterization of xylanase producing fungi, optimization of medium composition and cultural conditions for xylanase enzyme production using cheaper sources. The fungal strains were isolated from garden soil by serial dilution technique and Aspergillus niger was identified and isolated in pure form. In conformation screenin...

The present study deals with the isolation, screening and selection of native fungal strain for the alpha amylase production. Forty fungal strains were isolated from different soil samples. These strains were initially screened qualitatively on starch agar medium and quantitative screening was carried out in solid state fermentation. A strain of Aspergillus niger showing maximum production (432...

The efficacy of slow filters for horticultural purposes is suggested to be the result of multiple factors. Physical and biological properties of the filter column and the nutrient solution are likely to influence on filter performance. It has previously been shown that enzyme activity on the filter skin is a main parameter which will explain roughly 50% of filter efficacy. Since enzyme activity...

Most commercially available electrochemical enzyme sensor strips for the measurement of blood glucose use an artificial electron mediator to transfer electrons from the active side of the enzyme to the electrode. One mediator recently gaining attention for commercial sensor strips is hexaammineruthenium(III) chloride. In this study, we investigate and compare the preference of enzyme electrodes...

Dermatophyte and yeast species have been screened for their degradative ability towards various keratin substrates. Application of soluble preparation of keratin (KS) of chicken feathers enables a preliminary evaluation of the growth of the fungi and screening of fungal isolates that possesed keratinolytic activity and keratinase enzyme. Five dermatophytes i.e. Trichophyton rubrum, Trichophyton...

The X-ray structure of chitinase from the fungal pathogen Coccidioides immitis has been solved to 2.2 A resolution. Like other members of the class 18 hydrolase family, this 427 residue protein is an eight-stranded beta/alpha-barrel. Although lacking an N-terminal chitin anchoring domain, the enzyme closely resembles the chitinase from Serratia marcescens. Among the conserved features are three...

Tomatinase, an extracellular enzyme belonging to family 3 of the glycosyl hydrolases, is produced by the fungal tomato-leaf pathogen Septoria lycopersici and detoxifies the saponin alpha-tomatine. An efficient strategy for purification of the enzyme from fungal culture medium has been developed. Single crystals have been grown by vapour diffusion at 289 K from 17.5%(w/v) PEG 4K, 5%(v/v) 2-propa...

Azoles used for invasive fungal infections include two classes, the imidazoles (ketoconazole) and the triazoles (itraconazole, fluconazole, voriconazole, posaconazole). A number of agents are available for topical use in different indications but this is beyond the paper’s focus. Azoles are usually recognized according to spectrum of activity and safety profiles. The imidazole ring is the synth...