Obtaining sexed sperm from previously frozen doses (reverse-sorted semen [RSS]) provides an important advantage because of the possibility of using the semen of bulls with desired genetic attributes that have died or have become infertile but from whom frozen semen is available. We report the efficiency of RSS on the pregnancy rate and birth rate of calves in a large-scale program using ovum pi...

Eriksson, B. 2000. Cryopresewation of boar semen. Studies on sperm viability in vitro and fertility. Doctoral thesis. ISSN 1401-6257. ISBN 91-576-5903-6 The world’s pig population is consistently being upgraded through the international trade of superior genetics. The two major systems that are used for this purpose are the transport of live animals and the export of frozen boar semen. The main...

Basic concepts of cryopreservation and the causes of cryoinjury are reviewed. The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane. Modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are also briefly discussed.

The ability to ship cooled stallion sperm for subsequent freezing at a facility specializing in cryopreservation would be beneficial to the equine industry. Stallion sperm has been centrifuged, cooled to 5 degrees C for 12 h, and frozen without a detrimental effect on motility in a previous study; however, no fertility data were available. Experiment 1 compared the post-thaw motility of sperm c...

The methodologies used for cytometric sorting of fresh spermatozoa never allowed a clear resolution of sexual chromosomes of frozen-thawed semen. To devise a novel method for the production of bovine predefined sexed embryos using frozen-thawed semen, sorting efficiency of different protocols was studied using a new quantitative real-time PCR method to verify the purity of sexed semen. To this ...

There are various advantages, such as the preservation of genetic resources, an improvement of the reproduction performance in summer, long-distance transportation of semen and the possibility of progeny test, in the cryopreservation technique of boar semen. However, cryopreserved boar spermatozoa have not been routinely available to swine artificial insemination (AI) due to the lower conceptio...

Artificial Insemination (AI) involves the introduction of sperm into the reproductive tract of the mare without natural mating. AI in the horse was first practiced long ago. Ancient Arabian texts describe how mares were successfully inseminated. In the late 18th Century, an Italian scientist took the idea up again. He performed a series of studies in which he placed stallion semen in the snow a...

The aim of the study was to evaluate motility parameters of Lacaune (Lc) and east-Friesian (eF) ram frozen-thawed spermatozoa by cASA (computer-Automated Sperm Analysis) technique in relation to their in vivo fertilizing ability. Fresh ejaculates were diluted in a Triladyl extender containing 20 % egg yolk, 6 % glycerol and 0.1 % trehalose and frozen in a liquid nitrogen vapour. The evaluation ...

Supplementation of semen extender with caffeine and CaCl(2) for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl(2) to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cerv...

Bovine Leukemia ProVirus: Evidence of Presence of Part of Gag Gene in Seminal Plasma of Naturally Infected Bulls It is of critical importance to understand the modalities of BLV presence in semen, especially with regard to artificial insemination (AI). Presence of bovine leukemia provirus was demonstrated in fresh and frozen semen samples by researchers. In this study paired blood and semen sam...