OBJECTIVE During IVF, non-transferred embryos are usually selected for cryopreservation on the basis of morphological criteria. This investigation evaluated an application for array comparative genomic hybridization (aCGH) in assessment of surplus embryos prior to cryopreservation. METHODS First-time IVF patients undergoing elective single embryo transfer and having at least one extra non-tra...

Many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. Previous studies suggested that growth factors such as epidermal growth factor (EGF) are important in pre-implantation embryo development and implantation process. On the other hand, it is very important to support post thaw development of frozen em...

background: frozen- thawed embryo transfer is an essential part of art treatment and outcomes of this procedure are associated with several clinical factors. several studies have showed an increase level of ivf outcomes in women with sufficient vitamin d. objective: whether treatment of vitamin d insufficiency can improve pregnancy rates in frozen-thawed embryo transfer cycles. materials and me...

BACKGROUND Abnormalities of oocyte morphology affect embryo quality and viability. Whether morphological abnormalities of the oocyte influence cryosurvival and further development of derived embryos is not known. The aim of this study was to compare cryosurvival and progression to the blastocyst stage of frozen-thawed embryos derived from normal and abnormal oocytes. METHODS A total of 5292 G...

many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. previous studies suggested that growth factors such as epidermal growth factor (egf) are important in pre-implantation embryo development and implantation process. on the other hand, it is very important to support post thaw development of frozen em...

background: the purpose of this study was to determine the optimal endometrial preparation protocol by comparing the clinical outcome of two methods of endometrial preparation in frozen-thawed embryo transfer (fet) cycles, including that is, oral estradiol and 17ß-estradiol transdermal patch. methods: in this randomized controlled trial, women underwent either conventional ivf or intracytoplasm...

At present, the problem of increasing effectiveness programs assisted reproductive technologies and successful infertility treatment is still relevant. Assisted hatching used in devitrificated embryo transfer facilitates exit from pellucide zone. Yet clinical efficacy relevant debatable. There are no clear indications for use this technology, groups patients have been identified. The aim study....

Study question: Does the type of medium used to culture fresh and frozen-thawed embryos influence neonatal birthweight after single embryo transfer (SET) in IVF? Summary answer: A comparison of two commercially available culture media showed no significant influence on mean birthweight and mean birthweight adjusted for gestational age, gender and parity (z-scores) of singletons born after a fre...

BACKGROUND Recipients of donor oocytes need to be synchronized to the donor's cycle if fresh embryos are to be transferred on the cycle of oocyte retrieval. It would be much easier to merely retrieve the oocytes from the donor, fertilize the oocytes with the recipient's male partner's spermatozoa, cryopreserve the embryos, then transfer on an oestrogen/progesterone treatment programme. METHOD...

A total of 410 goat embryos were divided at random into 9 groups. Cryoprotectants (glycerol, ethylene glycol or dimethylsulfoxide) were added by a 3-step procedure using increasing concentrations of cryoprotectant (0.5 M; 1 M; 1.5 M) in PBS at 10 min intervals. After freezing and thawing, each cryoprotectant was removed by 3 methods: the classic 3-step procedure (cryoprotectant 1 M-10 min; 0.5 ...