Twentieth-century researcher Ernest John Christopher Polge studied the reproductive processes of livestock and determined a method to successfully freeze, thaw, and utilize viable [4] sperm [5] cells to produce offspring in animals. In 1949, Polge identified glycerol as a cryoprotectant, or a medium that enables cells to freeze without damaging their cellular components or functions. Several ye...

We tested the hypotheses that freezing-induced embolism is related to conduit diameter, and that conifers and angiosperms with conduits of equivalent diameter will exhibit similar losses of hydraulic conductivity in response to freezing. We surveyed the freeze-thaw response of conifers with a broad range of tracheid diameters by subjecting wood segments (root, stem and trunk wood) to a freeze-t...

This paper explains the procedure and results of an experiment on the relationship between the unconfined compressive strength and percent weight loss due to freeze and thaw durability test of laboratory compacted soil-cement specimens. The tests were carried out on soil cement mixtures of 10 different soil types. It was shown that the percent weight loss due to freeze and thaw durability test ...

The soil column samples were collected for indoor simulated freeze-thaw experiments to monitor the hydrothermal dynamics and measure basic physicochemical properties research effects of on process peat bog its relationship with properties. results indicate that in initial phase freezing-thawing, water content decreases temperature changes, respectively. Unfrozen stable freezing period sharply. ...

AIM The aim of this study was to investigate the effect of commercial egg yolk powder as an alternative to fresh egg yolk on freezability of Murrah buffalo semen. MATERIALS AND METHODS Semen samples (12) from 3 Murrah buffaloes (4 from each bull) with mass motility (≥3+) and total motility (70% and above) were utilized in this study. Immediately after collection, each sample was divided into ...

The soil samples of the roadbed slope Datong-Xi'an high-speed railway section are taken as research object. frost-heave property fiber under a single freeze-thaw cycle is studied, where fiber's content into account. distributions temperature and humidity fields inside numerically coupling transfer heat moisture revealed. results helpful for optimal design

Background: Sperm storage via freezing has been useful for men who have difficulty masturbating during assisted reproductive technology (ART) programs and before impotency caused by chemotherapy, vasectomy, and other procedures. Studies were undertaken to evaluate the extent of cryoinjury to both semen and sperm after repeated freezing and thawing in oligozoospermic and normozoospermic samples ...