this study was conducted to evaluate the effects of freeze-thaw cycles on some quality parameters of silver carp fillets. the prepared fillets were packaged in zip lock bags and were subjected to 0, 1, 2, 3, 4 and 5 freeze-thaw cycles, respectively. heme iron content decreased, and dry matter increased as the number of freeze-thaw cycles increased (p

Lactic acid bacteria (LAB) and probiotics promise specific health benefits to their host. However, good storage stability is a prerequisite for functioning industrial use. This study aimed evaluate glutathione (GSH) as potential protective agent improving microbial deteriorated by freeze-drying, freeze-thawing, cold treatments. In this study, the optimal concentration of (50% w/w) was 1%, showi...

To demonstrate the adaptation of lactic acid bacteria to environmental stresses can improve cell viability during freeze-drying, Lactobacillus plantarum VAL6 strain was cultured under different stress conditions, including temperature, pH, and increase in CO2 concentration. The results densitometry analysis showed that able survive harsh conditions such as at pH 2.5, temperature 47oC, anaerobic...

C-Phycocyanin (PC) is a protein used commercially as natural blue pigment produced by cyanobacteria, cryptophytes, and rhodophytes. Although it industrially synthesized from the cyanobacterium Arthrospira platensis, PC requires high levels of energy for its extraction, which involves freezing cells. However, protein, easily denatured at extreme temperatures. In this study, we extracted red alga...

The majority of overwintering insects avoid lethal freezing by lowering the temperature at which ice spontaneously nucleates within their body fluids. We examined the effect of ice-nucleating-active bacteria on the cold-hardiness of the lady beetle, Hippodamia convergens, a freeze-intolerant species that overwinters by supercooling to ca. -16 degrees C. Topical application of the ice-nucleating...

Bifidobacterium longum and Streptomyces peucetius are two totally different bacterial species with respect to environmental conditions and process objective. These were used as model organisms in this study. The objective of the B. longum process was to produce viable biomass suitable for use as food additive. The objective of the S. peucetius process was to produce a medically significant comp...

approved: ____________________________________________________________ Adam Z. Higgins Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any future RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s abil...

Alkaline phosphatase (ALP) is the most widely recognized biochemical marker for osteoblast activity. Although its precise function is poorly understood, it is believed to play a role in skeletal mineralization. The aim of this study was to develop an assay suitable for measuring the activity of this enzyme in microtiter plate format. Using the well-characterized osteoblast-like cell line Saos-2...

Abstract—Lyophilization, also called freeze-drying, is an important dehydration technique mainly used for pharmaceuticals. Food industry also uses lyophilization when it is important to retain most of the nutritional quality, taste, shape and size of dried products and to extend their shelf life. Vacuum-Induced during freezing cycle (VI) has been used in order to control ice nucleation and, con...

Measurement of fecal cytokines has been used as a marker of intestinal inflammation in people and correlates with endoscopic findings. The aim of this study was to evaluate the use of canine-specific enzyme-linked immunosorbant assays (ELISAs) for quantification of cytokines in canine fecal samples as a non-invasive biomarker. Interleukin (IL)-6, -8, -10, -23/12p40 and TNF- were assessed by us...