AIM To evaluate the efficiency of phenol/chloroform, microwave, and Qiagen spin column based DNA extractions from paraffin wax embedded tissue for use in the polymerase chain reaction (PCR). In addition, to assess the reliability of amplifying a housekeeping gene to indicate successful viral DNA extraction. METHODS DNA samples extracted from 20 blocks of cervical carcinoma tissues using the t...

The research aimed at developing an optimal procedure for the extraction of soybean saponins from lowtemperature soybean meal with microwave treatment combined with enzymatic hydrolysis (MTEH), and studied the antioxidant activity of soybean saponins. The result shows that the optimal parameters of microwave treatment, determined with the orthogonal array design method, were the medium fire of ...

The objective of this research was to develop, optimize, and validate a modern, rapid method of preparation of human hair samples, using microwave irradiation, for analysis of eight tricyclic antidepressants (TCADs): nordoxepin, nortriptyline, imipramine, amitriptyline, doxepin, desipramine, clomipramine, and norclomipramine. It was based on simultaneous alkaline hair microwave-assisted hydroly...

Microwave assisted extraction technique was employed to extract pectin from citron peel. Box-Behnken design was applied to investigate the influence of irradiation time, microwave power and pH on the yield and DE of pectin. The finding indicated that the optimal conditions for the maximum yield of pectin (30.71%) were achieved at irradiation time of 3 min, microwave power of 700 W and pH of 1.5...

In this study, microwave-assisted extraction was applied for pectin extraction from the dried orange peel and Box-Behnken response surface design was used to study and optimize the effects of processing variables (microwave power, irradiation time, pH and solid-liquid ratio) on the yield of pectin. The amount of pectin extracted increased with increasing microwave power, while it reduces as the...

Melamine trisulfonic acid (MTSA) is utilized as a highly efficient catalyst for the solvent-free condensation reaction of 2-naphthol (2 eq.) with arylaldehydes (1 eq.) under conventional thermal as well as microwave conditions to give the d

Deep eutectic solvents (DESs) have attracted significant attention as a promising green media. In this work, twenty-five kinds of benign choline chloride-based DESs with microwave-assisted methods were applied to quickly extract active components from Radix Salviae miltiorrhizae. The extraction factors, including temperature, time, power of microwave, and solid/liquid ratio, were investigated s...

Present study was based upon the phytochemical screening, antimicrobial and antithrombotic potential of a traditional herb Spharenthus indicus (commonly called East Indian globe thistle). A range of solvents from non-polar to polar (n-hexane, ethyl acetate, methanol) were used to attained the extract by employing conventional and modern methods of extraction like orbital shaker assisted extract...

We use conventional, microwave and ultrasound assisted sequential extraction, of defined time and power, techniques for extractions of Cd, Cu, Cr, Ni, Pb and Zn in sediments and certified material. We did not change the conditions of extractions through steps, cause we what to see is there difference in extraction results for the certified material and sediments. We use lower powers and time fr...