Conventional biochemical analysis mainly focuses on the expression level of cellular proteins from entire cells. However, it has been increasingly acknowledged that the subcellular location of proteins often carries important information. Analysis of subcellular proteins conventionally requires subcellular fractionation which involves two steps: cell lysis to release proteins and high-speed cen...

1. Solubility curves of crude pepsin preparations indicate the presence of more than one protein. 2. One of these proteins has been isolated and crystallized and found to have constant activity and constant solubility in several solvents. 3. The solubility measurements are complicated by the unstable nature of the protein and the fact that in certain solvents the solubility of the protein is ma...

Mg in two Allende Ca-AI rich inclusions shows large isotopic, mass-dependent fractionation which enriched the heavier isotopes. After normalization, Mg in these inclusions shows negative 6 16 Mg which appears to require the presence of nuclear effects m Mg distinct from 16 AI decay. The Mg mass fractionation is correlated with distinct but smaller fractionation effects for 0 reported by Clayton...

CONTEXT The major motivation for the preparation of the plasma derived biological medicine was the treatment of casualties from the Second World War. Due to the high expenses for preparation of plasma derived products, achievement of self-sufficiency in human plasma biotechnological industry is an important goal for developing countries. EVIDENCE ACQUISITION The complexity of the blood plasma...

density lipoproteins in normal humans. J Lipid Res 1982;23:97–104. 6. Scheffer PG, Bakker SJL, Heine RJ, Teerlink T. Measurement of LDL particle size in whole plasma and serum by high-performance gel-filtration chromatography using a fluorescent lipid probe. Clin Chem 1998;44:2148–51. 7. Teerlink T, Scheffer PG, Bakker SJL, Heine RJ. Combined data from LDL composition and size measurement are c...

The nuclear volume component of equilibrium field shift isotope fractionations in europium and other lanthanide elements is estimated using Mössbauer spectroscopy electronic structure calculations. This effect goes the opposite direction from mass-dependent fractionation, case predicted to dominate over mass dependent fractionation for most materials. Including both effects, Eu2+-bearing specie...

ABSTRACT: In the present study, Persian gum, an exudate gum which secrets from the mountain almond tree (Amygdalus scoparia Spach) from two different regions is evaluated by means of chemical and instrumental analysis (fractionated by solving in water and alkaline solutions at 0.1, 0.5 and 1 M and also Smith degradation) using HPLC, 1H–NMR and 13C–NMR spectroscopy’s. The total sugar and uronic ...