A substance which was mitogenic for murine B lymphocytes in the presence of 2-mercaptoethanol was isolated from agar. Stimulating activity of this material was stable to proteolysis or protein denaturants but was destroyed by periodate treatment. Agar-derived mitogen stimulation was distinct from that obtained with dextran sulfate or lipopolysaccharide and may define different populations of B ...

Flagella act as semirigid helical propellers that are powered by reversible rotary motors. Two membrane proteins, MotA and MotB, function as a complex that acts as the stator and generates the torque that drives rotation. The genome sequence of Pseudomonas aeruginosa PAO1 contains dual sets of motA and motB genes, PA1460-PA1461 (motAB) and PA4954-PA4953 (motCD), as well as another gene, motY (P...

Magic-angle sample spinning is one of the cornerstones in high-resolution NMR of solid and semisolid materials. The technique enhances spectral resolution by averaging away rank 2 anisotropic spin interactions, thereby producing isotropic-like spectra with resolved chemical shifts and scalar couplings. In principle, it should be possible to induce similar effects in a static sample if the direc...

Five distinct bacteriophages have been isolated from strains of Neisseria meningitidis. Filtrates with titers of 10(-4) to 10(-6) were produced with a modified Swanstrom and Adams semisolid agar procedure, employing Eugonbroth with added agar and an incubation temperature of 30 C. Of 49 strains of N. meningitidis (groups B and C), 25 were lysed by one or more of the phages, but there was no lys...

a week. On the day of fourth injection, when I gave him 0.1 gramme, suddenly he complained of severe abdominal pain and pressed his hand to his abdomen and after a couple of minutes he became unconscioushis breathing was very hard, pulse was very slow an" almost imperceptible and saliva was coming out of his mouth profusely. I was very much alarmed at these symptoms of the patient. I immediatel...

The effect of reheating to the semisolid state (soaking treatment) on the microstructure evolution of the A356 aluminum alloy prepared by ultrasonic melt treatment was studied in this paper. The results showed that in general the longer the soaking process the larger and the more round the grains obtained. Higher roundness occurs at shorter soaking times in the fine-grained ascast samples, and ...

A three-phase study was conducted to compare Hektoen enteric agar (HE), Rambach agar (Ra), SM-ID medium (SM), xylose-lysine-Tergitol 4 agar (XLT4), novobiocin-brilliant green-glycerol-lactose agar (NBGL), and modified semisolid Rappaport-Vassiliadis medium (MSRV) for the recovery of nontyphoid salmonellae from stool specimens. After evaluation of the first two phases, which resulted in the elim...

Our results show that all of the brown rot fungi tested produce oxalic acid in liquid as well as in semisolid cultures. Gloeophyllum trabeum, which accumulates the lowest amount of oxalic acid during decay of pine holocellulose, showed the highest polysaccharide-depolymerizing activity. Semisolid cultures inoculated with this fungus rapidly converted C-labeled oxalic acid to CO(2) during cellul...

Frameshift mutations in a poly(G) track at the flhB gene of Pseudomonas putida DOT-T1E are responsible for the diminished swimming of this strain on semisolid medium, which contrasts with the high swimming ability of P. putida KT2440, which does not exhibit a poly(G) track at the flhB gene. We previously showed that a mutant lacking FlhB was more sensitive to solvents than the wild-type strain ...

The effect of repeated temperature variations on the performance of both fresh and aged dye-sensitized solar cells with liquid and semisolid electrolytes has been studied. The cell performance was characterized with IV-curves obtained at different cell operating temperatures and electrochemical impedance spectroscopy measurements before and after the temperature treatments. Consecutive temperat...