PURPOSE We aim to fabricate a thermoplastic poly(methylmethacrylate) (PMMA) Lab-on-a-Chip device to perform continuous- flow polymerase chain reactions (PCRs) for rapid molecular detection of foodborne pathogen bacteria. METHODS A miniaturized plastic device was fabricated by utilizing PMMA substrates mediated by poly(dimethylsiloxane) interfacial coating, enabling bonding under mild conditio...

Foods of non-animal origin (FoNAO) are consumed in a variety of forms, being a major component of almost all meals. These food types have the potential to be associated with large outbreaks as seen in 2011 associated with VTEC O104. In order to identify and rank specific food/pathogen combinations most often linked to human cases originating from FoNAO in the EU, a semi-quantitative model was d...

The rapid high-throughput detection of foodborne pathogens is essential in controlling food safety. In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was established for the rapid and simultaneous detection of 10 epidemic foodborne pathogens. Ten different single-target UPT-LF strips were developed and integrated into one TC-UPT-LF disc with opti...

The Centers for Disease Control and Prevention (CDC) recently revised their estimates for the annual number of foodborne illnesses; 48 million Americans suffer from domestically acquired foodborne illness associated with 31 identified pathogens and a broad category of unspecified agents. Consequently, economic studies based on the previous estimates are now obsolete. This study was conducted t...

Foodborne infection is a result of exposure to complex, dynamic food systems. The efficiency of foodborne infection is driven by ongoing shifts in genetic machinery. Next-generation sequencing technologies can provide high-fidelity data about the genetics of a pathogen. However, food safety surveillance systems do not currently provide similar high-fidelity epidemiological metadata to associate...

Each year in the United States, foodborne diseases affect millions of persons, who become ill after exposure to any of a growing spectrum of identified agents and toxins. Typhoid fever and other foodborne diseases common a century ago have been controlled by measures that prevent contamination of food and water with human sewage and by technologies (such as milk pasteurization) that eliminate a...

Non-typhoidal Salmonella species are important foodborne pathogens that can cause gastroenteritis, bacteremia, and subsequent focal infections. Non-typhoidal salmonellosis is problematic, particularly in immunocompromised hosts. Any anatomical site can be affected by this pathogen via hematogenous seeding and may develop local infections. However, cervical lymphadenitis caused by non-typhoidal ...

Aeromonas was classified previously with Vibrio species and Pleisomonas shigelloides in the Vibrionaceae [1]. Based on molecular genetic evidence, Aeromonas species is assigned separate family, Aeromonadaceae[1,2] . The genus aeromonas had been divided into 2 typesmesophilic which are potent human pathogens and psychrophilic consisting of non motile Aeromonas salmonicida [3]. Aeromonas verionii...

Foodborne disease is a global threat that can affect all sections of society, both in developed or developing countries . Bacillus cereus Gram-positive bacteria cause food poisoning humans. [2] Real-Time PCR detection method one the molecular marker methods has been widely recognized as fast, reliable, sensitive and specific tool for detecting pathogenic bacteria. In previous studies, optimum c...

Staphylococcus aureus, as a foodborne pathogen causing significant harm worldwide, was studied to assess the effectiveness of probiotic strains Lactobacillus casei, plantarum, and Bifidobacterium bifidum individually collectively (as consortia) in controlling its growth. The growth patterns S. aureus were observed when co-cultured with each strain consortium all three over 72 hours. Additionall...