Isoelectric Focusing in Immobilized pH Gradient Strips Using the IPGphor Unit: Sample In-Gel Rehydration Angelika Görg, Oliver Drews, and Walter Weiss This protocol was adapted from "IEF in IPG Strips Using the IPGphor Unit," contributed by Angelika Görg, Oliver Drews, and Walter Weiss, Chapter 16, in Purifying Proteins for Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spr...

Protein solubilization for two-dimensional electrophoresis (2DE) has to break molecular interactions to separate the biological contents of the material of interest into isolated and intact polypeptides. This must be carried out in conditions compatible with the first dimension of 2DE, namely isoelectric focusing. In addition, the extraction process must enable easy removal of any nonprotein co...

A modified method of Boyum's technique to isolate granulocytes and lymphocytes from human blood is described. The granulocytes and the lymphocytes separated by this isolation procedure are highly pure, viable, and in sufficient number for subsequent isoelectric focusing studies. Different proteins have been separated by isoelectric focusing. This technique can be useful in the investigation of ...

We have modified one of the most useful methods of protein separation; namely, two dimensional gel electrophoresis (2-DE). This modified version of 2-DE is not only simpler and easier but also faster than all the currently available methods. In this method, isoelectric focusing is carried out in the first dimension using a vertical sodium dodecyl sulfate polyacrylamide gel electrop...

Free flow electrophoresis (FFE) is a fractionation method, based on isoelectric focusing (IEF). We validate the reproducibility of the method and show that it can be applied by biobanks in order to fractionate fluid biospecimens efficiently and reproducibly and to facilitate downstream proteomic applications. We also propose a simple method allowing researchers to assess the reproducibility of ...

We discuss two fast algorithms to localize multiple sources in near field. The symmetry-based method proposed by Zhi and Chia (2007) is first improved by implementing a search-free procedure for the reduction of computation cost. We present then a focusing-based method which does not require symmetric array configuration. By using focusing technique, the near-field signal model is transformed i...

background and aims: influenza virus nucleoprotein (np) has the capacity to be used as subunit vaccine, but little is known about the impact of different cultures on its structure. in the present study we aimed to evaluate and compare the isoelectric focusing (ief) property of extracted viral nucleoproteins derived from madin darby canine kidney (mdck) cell line and embryonated chicken eggs (ec...

Isoelectric focusing on ultrathin polyacrylamide foils, coupled with isoenzymatic analysis using five different enzymes, was used to characterize blood-forms of T.b. brucei, T.b. rhodesiense and T.b. gambiense and procyclic forms of T.b. brucei and T.b. rhodesiense. The protein concentrations in the lysates were quantified. Qualitative as well as quantitative differences were found.