نتایج جستجو برای: fluorescent in situ hybridization

تعداد نتایج: 16994172  

Journal: :Molecular and cellular biology 2010
Eladio Abreu Elena Aritonovska Patrick Reichenbach Gaël Cristofari Brad Culp Rebecca M Terns Joachim Lingner Michael P Terns

Recruitment to telomeres is a pivotal step in the function and regulation of human telomerase; however, the molecular basis for recruitment is not known. Here, we have directly investigated the process of telomerase recruitment via fluorescence in situ hybridization (FISH) and chromatin immunoprecipitation (ChIP). We find that depletion of two components of the shelterin complex that is found a...

Journal: :Science 2007
Mario Ventura Francesca Antonacci Maria Francesca Cardone Roscoe Stanyon Pietro D'Addabbo Angelo Cellamare L James Sprague Evan E Eichler Nicoletta Archidiacono Mariano Rocchi

A systematic fluorescence in situ hybridization comparison of macaque and human synteny organization disclosed five additional macaque evolutionary new centromeres (ENCs) for a total of nine ENCs. To understand the dynamics of ENC formation and progression, we compared the ENC of macaque chromosome 4 with the human orthologous region, at 6q24.3, that conserves the ancestral genomic organization...

Journal: :Methods 2015
Brian Munsky Zachary Fox Gregor Neuert

The production and degradation of RNA transcripts is inherently subject to biological noise that arises from small gene copy numbers in individual cells. As a result, cellular RNA levels can exhibit large fluctuations over time and from one cell to the next. This article presents a range of precise single-molecule experimental techniques, based upon RNA fluorescence in situ hybridization, which...

Journal: :Applied and environmental microbiology 2004
Maria Vila Rafel Simó Ronald P Kiene Jarone Pinhassi José M González Mary Ann Moran Carlos Pedrós-Alió

The fraction of planktonic heterotrophic bacteria capable of incorporating dissolved dimethylsulfoniopropionate (DMSP) and leucine was determined at two coastal sites by microautoradioagraphy (AU). In Gulf of Mexico seawater microcosm experiments, the proportion of prokaryotes that incorporated sulfur from [(35)S]DMSP ranged between 27 and 51% of 4',6-diamidino-2-phenylindole (DAPI)-positive ce...

2014
Pablo Reyes-Gutierrez Joan C Ritland Politz Thoru Pederson

We previously discovered that a set of 5 microRNAs are concentrated in the nucleolus of rat myoblasts. We now report that several mRNAs are also localized in the nucleoli of these cells as determined by microarray analysis of RNA from purified nucleoli. Among the most abundant of these nucleolus-localized mRNAs is that encoding insulin-like growth factor 2 (IGF2), a regulator of myoblast prolif...

2013
Jan Jezbera Jitka Jezberová Vojtěch Kasalický Karel Šimek Martin W. Hahn

Among abundant freshwater Betaproteobacteria, only few groups are considered to be of central ecological importance. One of them is the well-studied genus Limnohabitans and mainly its R-BT subcluster, investigated previously mainly by fluorescence in situ hybridization methods. We designed, based on sequences from a large Limnohabitans culture collection, 18 RLBH (Reverse Line Blot Hybridizatio...

Journal: :Environmental microbiology reports 2011
Mehdi Boutrif Marc Garel Matthew T Cottrell Christian Tamburini

This study examined total uptake of extracellular polymeric substances (EPS) and glucose and the percentage of prokaryotic cells (Bacteria, Crenarchaea and Euryarchaea) consuming these compounds in the major water masses at the DYFAMED site (NW Mediterranean Sea). The potential assimilation rates of EPS at 10 m depth were higher but on the same order of magnitude as those at 2000 m depth (from ...

Journal: :Haematologica 2008
Stephanie Struski Laurent Mauvieux Carine Gervais Catherine Hélias Kun Lun Liu Michel Lessard

The ETV6/GOT1 fusion, resulting from t(10;12) (q24;p13), has been recently described in a myelodysplastic syndrome. We reported a second case of t(10;12)-positive myelodysplastic syndrome in whom fluorescent in situ hybridization confirmed the non-random translocation but molecular biology analyses revealed a ETV6/GOT1 chimera varying from the first case described.

Journal: :Diagnostics 2016
Shivang Doshi David Ray Karen Stein Jie Zhang Prasad Koduru Franz Fogt Axel Wellman Ricky Wat Charles Mathews

Identification of alterations in ALK gene and development of ALK-directed therapies have increased the need for accurate and efficient detection methodologies. To date, research has focused on the concordance between the two most commonly used technologies, fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC). However, inter-test concordance reflects only one, albeit importan...

Journal: :Journal of microbiological methods 2007
J Bertaux U Gloger M Schmid A Hartmann S Scheu

The use of fluorescence in situ hybridization (FISH) to identify and enumerate soil bacteria has long been hampered by the autofluorescence of soil particles masking the bacterial signals and because the need of counting hundreds of bacteria in order to achieve statistically reliable data is time consuming. Recently, it was demonstrated that Nycodenz facilitates FISH in soil by concentrating ba...

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