One of the major quality assurance (QA) goals in many molecular laboratories is to avoid sample pipetting errors on the lab bench; especially when pipetting into multiwell plates. A pipetting error can cause a switch in patient samples, which can lead to recording the wrong results for the patient samples involved. Such pipetting errors are difficult to identify when it happens in lab bench wor...

Auranofin, a transition metal complex is used for the treatment of rheumatoid arthritis but is also an effective anti-cancer drug. We investigate the effects of Auranofin in inducing cell death by apoptosis and whether these changes are correlated to changes of intracellular calcium concentration ([Ca2+]i) in breast cancer cells (MCF-7). Cytotoxicity of Auranofin was evaluated using MTS assay a...

Aflatoxins difuranocoumarin derivatives are produced by fungi Aspergillus flavus, Aspergil‐ lus parasiticus and Aspergillus nomius [1] and they are part of the group of mycotoxins. From the twenty metabolites that have been formed endogenously in animals, aflatoxins B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2) are the most common and the most toxic. The names of aflatoxins B1, B2, G1, and G2 a...

This paper describes the characterization and leaching behaviour of precious metal present from electronics scrap to obtain liquor which can be treated through hydrometallurgical techniques to purify the metals. The results of this study points out the technical feasibility of the recovery of the metals. The blank sample was characterized by X-ray florescence. This paper will also present the r...

The NASA Soil Moisture Active Passive (SMAP) mission Level 4 Carbon (L4_C) product provides model estimates of Net Ecosystem CO2 exchange (NEE) incorporating SMAP soil moisture information as a primary driver. The L4_C product provides NEE, computed as total respiration less gross photosynthesis, at a daily time step and approximate 14-day latency posted to a 9-km global grid summarized by plan...

The insulin receptor tyrosine kinase substrate p53 (IRSp53) links Rac and WAVE2 and has been implicated in lamellipodia protrusion. Recently, however, IRSp53 has been reported to bind to both Cdc42 and Mena to induce filopodia. To shed independent light on IRSp53 function we determined the localisations and dynamics of IRSp53 and WAVE2 in B16 melanoma cells. In cells spread well on a laminin su...

Three different types of molecular markers, RAPD, SSR and fluorescence-based AFLP, were evaluated and compared for their ability to identify oilseed rape cultivars. The direct comparison of RAPD, SSR and AFLP approaches in cultivar identification showed that the AFLP methodology detected polymorphisms more efficiently than either RAPD or SSR methods. For the characterisation of six oilseed rape...

Mycoplasma hyorhinis (M. hyorhinis) is an opportunistic pathogen of pigs and has been shown to transform cell cultures, which has increased the interest of researchers. The green florescence proteins (GFP) gene of Aquorea victoria, proved to be a vital marker to identify transformed cells in mixed populations. Use of GFP to observe gene transfer and expression in M. hyorhinis (strain HUB-1) has...

BACKGROUND Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE The aim of this investigation was to assess the effect, in rats, of intratracheal instillation of particulate air pollution on leukocyte flow pro...