Members of the genus Actinoplanes are considered to be representative of motile actinomycetes. To infer the flagellar diversity of Actinoplanes species, novel degenerate primers were designed for the flagellin (fliC) gene. The fliC gene of 21 Actinoplanes strains was successfully amplified and classified into two groups based on whether they were large (type I) or small (type II). Comparison of...

background: infections due to pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. at present, aggressive antibiotic therapy is the only choice for management of p. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

the genus of salmonella is very polymorphic and comprised of a number of genetically closely related serotypes. it is one of the emerging pathogen in food-borne disease which is often found in contaminated chicken eggs. salmonella enterica is considered one of the major pathogens in public health worldwide. a total of 31 salmonella isolates identified by specific antisera, which included salmon...

To explore new targets for hepatoma research, we used a surface display library to screen novel tumor cell-specific peptides. The bacterial FliTrx system was screened with living normal liver cell line L02 and hepatoma cell line HepG2 successively to search for hepatoma-specific peptides. Three clones (Hep1, Hep2, and Hep3) were identified to be specific to HepG2 compared with L02 and other can...

The Fat-Link Irrelevant Clover (FLIC) fermion action provides a new form of nonperturbative O(a) improvement and allows efficient access to the light quark-mass regime. FLIC fermions enable the construction of the nonperturbatively O(a)-improved conserved vector current without the difficulties associated with the fine tuning of the improvement coefficients. The simulations are performed with a...

The role of flagella in the pathogenesis of F4ac+ Enterotoxigenic Escherichia coli (ETEC) mediated neonatal and post-weaning diarrhea (PWD) is not currently understood. We targeted the reference C83902 ETEC strain (O8:H19:F4ac+ LT+ STa+ STb+), to construct isogenic mutants in the fliC (encoding the major flagellin protein), motA (encoding the flagella motor), and faeG (encoding the major subuni...

Hadron masses are calculated in quenched lattice QCD in order to probe the scaling behavior of a novel fatlink clover fermion action in which only the irrelevant operators of the fermion action are constructed using APE-smeared links. Light quark masses corresponding to an mπ/mρ ratio of 0.35 are considered to assess the exceptional configuration problem of clover-fermion actions. This Fat-Link...