This paper describes a new method for qualitative and quantitative assay of abscisic acid and other acidic plant hormones, such as indoleacetic acid and the gibberellins, by the gas-liquid chromatography of their trimethylsilyl derivatives. Interfering substances in plant extracts were largely removed by preliminary column chromatography with carbon-celite and elution of the abscisic acid with ...

Menthol is widely used as a fragrance and flavor in the food and cosmetic industries. It is also used in the medical and pharmaceutical fields for its various biological effects. Gas chromatography (GC) is considered to be a sensitive method for the analysis of menthol. GC chromatographic separation was developed using capillary column (VF-624) and a flame ionization detector (FID). The method ...

The main effects of six experimental factors on the efficiency of HS (headspace) extraction in headspace gas chromatography--flame ionization detector (HS-GC-FID) determination of twenty organic solvents routinely used in production of pharmaceuticals were obtained on the basis of the results of experiments carried out according to the Plackett-Burman factorial design. The effects were used as ...

A capillary gas chromatography method using a flame ionization detector has been developed for the trace analysis of allylamine (AA) in sevelamer hydrochloride (SVH) and sevelamer carbonate (SVC) drug substances. The method utilized a mega bore capillary column DB-CAM (30 m × 0.53 mm × 1.0 μm) with a bonded and cross-linked, base-deactivated polyethylene glycol stationary phase and was validate...

A simple, precise, and accurate stability indicating gas chromatographic method has been developed and validated for assay of Magnesium Valproate. The Chromatographic system used to perform development and validation of this assay method consisted of Shimadzu 2014 G.C. with Flame Ionization Detector and a manual injector. The method was validated for specificity, linearity, precision, accuracy,...

ABSTRACT Oil blending is often used to enhance the properties of vegetable oils. The admixture of a more thermally stable oil makes the resulting blend more suitable for use in frying. A new method of quality assessment of vegetable oils used in frying is presented in this paper. In this method, ultra-fast gas chromatography coupled with flame ionization detector and chemometrics is employed. P...

The present work aimed to evaluate whether it is possible to use oral fluid to monitor alcohol in drivers. In a control experiment the subjects ingested beer with an alcoholic percentage of 4.7%, in an amount that furnished 0.5 g ethanol per kg of body weight. Volunteer's urine, oral fluid, and breath were collected at 10, 30, 60, and 90 min after alcohol intake. Urine and oral fluid were analy...

We report a micro-scale procedure for determination of theophylline by use of gas chromatography with nitrogen-sensitive detection. The procedure requires only 10 mul of serum, plasma, or saliva. This sample size makes the determination especially appropriate for monitoring in a pediatric population. With the sample volume used, background interference is equivalent to about 0.1 mg/liter and 0....

Lipid-based oleaginous microorganisms are potential candidates and resources for the sustainable production of biofuels. This study was designed to evaluate the performance of several alternative bio-based solvents for extracting lipids from yeasts. We used experimental design and simulation with Hansen solubility simulations and the conductor-like screening model for realistic solvation (COSMO...

A preparative gas chromatography (pGC) method was developed for the separation of volatile components from the methanol extract of Curcuma rhizome. The compounds were separated on a stainless steel column packed with 10% OV-101 (3 m × 6 mm, i.d.), and then, the effluent was split into two gas flows. One percent of the effluent passed to the flame ionization detector (FID) for detection and the ...