We present a simple method to resolve discrete intensity shifts from time-resolved single-molecule emission data. This new method uses multiples of the standard deviation of the measured intensities that are integrated into short time bins. By applying the technique to trimeric units of the main light-harvesting complex (LHCII) of plants, it is shown that the amount of information that can be e...

Redox-regulated reversible phosphorylation of the light-harvesting complex II (LHCII) controls the excitation energy distribution between photosystem (PS) II and PSI. The PsaL and PsaH subunits of PSI enable the association of pLHCII to PSI. Here, we show that the failure of the psal mutant to dock pLHCII to PSI induces excessive phosphorylation of LHCII, primarily due to a marked downregulatio...

The object of this study was evaluation of a walking tractor drawn peanut harvester at different conditions of soil moisture content and forward speed and comparing it with manual harvesting. The evaluation factors for peanut harvester were two levels of soil moisture content and three levels of forward speed. The results revealed that the effect of soil moisture content was only significant on...

Fish diversity and ecology in the ocean’s mesopelagic zone are understudied compared to other marine regions despite growing interest harvesting these potential resources. Otoliths can provide a wealth of taxonomic life history information about fish, which help fill knowledge gaps; however, there has been relatively little research date on otoliths species. Here, species-specific image library...

A bioenergetic growth model was developed to examine the integrated effects of fertilization, stocking density, and spawning on the growth of tiiapia, Oreochromis niloticus (L.), in pond aquaculture. The analyses showed that growth rates increase with higher levels of organic ferlilization up to 5(HJkg/ha/week. Growth rates increased with added food rations in ponds, reaching a maximum growth o...

The time-resolved fluorescence intensity fluctuations from single, immobilized complexes of the main light-harvesting complex (LHCII) of plants were investigated in different pH environments close to room temperature and under different light conditions. The efficiency of light harvesting, which was represented by complexes typically residing for long periods in strongly fluorescing states, was...

Ecosystems are usually complex, nonlinear and strongly influenced by poorly known environmental variables. Among these systems, marine ecosystems have high uncertainties: marine populations in general are known to exhibit large levels of natural variability and the intensity of fishing efforts can change rapidly. These uncertainties are a source of risks that threaten the sustainability of both...

Employing the utility of the native chemical ligation, site-specific attachment of an ultrastable perylene dye to a derivative of the major light-harvesting complex (LHCII) was demonstrated. Biochemical analysis of the conjugate indicated that the structure and function of LHCII remain largely unaffected by the N-terminal modification.

The COVID-19 pandemic is now spreading throughout the world affecting the agricultural activities including fish and shrimp culture sectors. Most of the shrimp producing countries particularly in South-east Asia have been affected due to the lockdown, quarantine roles and regulations ordered by the countries, which was assigned to reduce and control the COVID-19 pandemic spreading in the globe....