Patients and methods Fresh tumour samples from 27 CRC patients were used to prepare tumour spheroids. Autologous PBMCs were isolated by ficoll density gradient. Tumour spheroids co-cultured with or without PBMCs were treated with catumaxomab for 96h. The cellular composition of the spheroids and the therapeutic impact on epithelial cells and leukocytes were measured by FACS analysis. The metabo...

Normal spleen cells cultured with TNP-modified syngeneic spleen cells fail to mount an anti-TNP PFC response to TNP-ficoll or TNP-red blood cells,but go on to generate cytotoxic T cells directed at hapten-modified H-2.These results suggest that hapten-modifeid spleen cells may differentially induce B-cell tolerance and T- (Ly 2,3) cell immunity. The differential response to modified self by lym...

In the present study, we describe in vivo cultivation to produce oocysts. Seven-day-old mice were orally infected with 100,000-120,000 Cryptosporidium oocysts. On day 8 post-infection, the mice were killed by ether, and the small and large intestines collected. A simple extraction procedure was used and purified using Ficoll gradient centrifugation. After purification, the oocysts were preserve...

The respiratory control of rat liver mitochondria is lost when they are subjected to rate zonal centrifu~ation in a sucrose gradient (8.0% to 46.6%, w/w) at values for r t necessary for resolution. High sucrose concentrat ion and high ~ 2 t are bo th responsible. Respiratory control can be maintained in iso-osmotic Ficoll + 8.3% sucrose media, and after zonal centrifugation in such media at val...

The tetrameric manganese superoxide dismutase from the extreme thermophile Thermus thermophilus HB8 crystallizes in space group P41212 (or its enantiomorph) with a = b = 147.5 A, c = 55.9 A. The diffraction patterns extent to 1.4 A, and the crystals are very resistant to decay induced by x-irradiation. Measurements of the crystal density in Ficoll gradients are consistent with an asymmetric uni...

Treatment of lymphocytes with inducers of interferon a (IFN-a) results in the production of corticotropin (ACTH) and endorphin-like activities. The pro-opiomelanocortinderived hormones ACTH and a-, (-, and y-endorphin and the structurally related hormones [Leu]and [Metlenkephalin were therefore tested for their effects on the in vitro antibody response ofmouse spleen cells. ACTH and a-endorphin...

Noncoding RNAs must function in the crowded environment of the cell. Previous small-angle x-ray scattering experiments showed that molecular crowders stabilize the structure of the Azoarcus group I ribozyme, allowing the ribozyme to fold at low physiological Mg(2+) concentrations. Here, we used an RNA cleavage assay to show that the PEG and Ficoll crowder molecules increased the biochemical act...

Prolonged cultivation of separated rat lung mast cells (LMC) in vitro is necessary to better investigate a possible role of LMC in different stages of tissue remodeling induced by hypoxia. Rat lung mast cells (LMC) were separated using a protocol including an improved proteolytic extraction and two subsequent density gradient separations on Ficoll-Paque PLUS and a new generation of Percoll, i.e...

-Peripheral blood lymphocytes (PBL) were obtained from 13 patients and tumour-intrinsic lymphocytes (TIL) from 20 patients with colorectal cancer. The PBL were separated on a Ficoll-Isopaque gradient and the TIL by digestion of the tumour with collagenase-DNase. Both PBL and TIL were passed through nylonwool columns and the eluted cells were co-cultured for 2 h with 51Cr-labelled tumour cells f...