Plasminogen activation is catalyzed both by tissue-type(t-PA) and by urokinase-type plasminogen activator (u-PA). This reaction is controlled by plasminogen activator inhibitor type 1 (PAI-1) that is either present in plasma or bound to fibrin, present in a thrombus. We studied the mechanism of in vitro inhibition of both t-PA and u-PA activity by PAI-1 bound to fibrin. It is shown that activat...

Myosin modulates the fibrinolytic process as a cofactor of the tissueplasminogen activator and as a substrate of plasmin. We report now that myosin is present in arterial thrombi and it forms reversible non-covalent complexes with fibrinogen and fibrin with equilibrium dissociation constants in the micromolar range (1.70 and 0.94 μM, respectively). Competition studies using a peptide inhibitor ...

OBJECTIVES: Previous reports have revealed that several cytokines (including platelet-derived growth factor-BB, transforming growth factors-β1 and insulin-like growth factor-1) can enhance the rate of bone formation and synthesis of extracellular matrix in orthopaedics or periodontology. This study aimed to determine the concentration of cytokines within platelet-rich fibrin microstructures and...

Background . Due to the constant increase in number of surgeries restore blood flow affected vessels, development synthetic prostheses is relevant. One key success factors an adhesive properties inner surface, since rapid endothelialization vascular considered a factor necessary prevent thrombosis and neointimal hypertrophy. Aim : To determine effect surface modification polymer matrices with f...

Macro- and microvascular endothelial cells (EC) formed tubular structures when cultured within a 3D fibrin matrix, a process that was enhanced by vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2), hepatocyte growth factor/scatter factor (HGF/SF) and an angiogenic cocktail composed of nine angiogenic factors. Endothelial tubulogenesis was also increased in co-culture ...

Advanced atherosclerosis is often associated with dystrophic calcification, which may contribute to plaque rupture and thrombosis. In this work, the localization and association of the noncollagenous bone matrix proteins osteonectin, osteopontin, and osteocalcin with calcification, lipoproteins, thrombus/hemorrhage (T/H), and matrix metalloproteinases (MMPs) in human carotid arteries from endar...

Release of fibrinopeptide B from fibrinogen by copperhead venom procoagulant enzyme results in a form of fibrin ($-f,brin) with weaker self-aggregation characteristics than the normal product (a/9-fibrin) produced by release of fibrinopeptides A (FPA) and B (FPB) by thrombin. We investigated the ultrastructure of these two types of fibrin as well as that of $-fibrin prepared from fibrinogen Met...

Release of fibrinopeptide B from fibrinogen by copperhead venom procoagulant enzyme results in a form of fibrin ($-f,brin) with weaker self-aggregation characteristics than the normal product (a/9-fibrin) produced by release of fibrinopeptides A (FPA) and B (FPB) by thrombin. We investigated the ultrastructure of these two types of fibrin as well as that of $-fibrin prepared from fibrinogen Met...

background: connective tissue attachment following periodontal regenerative surgery is directly related to the attachment of fibrin clot on to the root surface during early wound healing events. the adhesion of fibrin clot to the root surface affected by periodontal disease depends on the biologic acceptance of the root surface which can be accomplished by various root conditioning procedures d...