The formation of a fibrin clot is one of the key events in atherothrombotic vascular disease. The structure of the fibrin clot and the genetic and environmental factors that modify it have effects on its biological function. Alterations in fibrin structure and function have implications for the clinical presentation of vascular disease. This review briefly describes the key features involved in...

We have discovered that addition of monomeric desAB fibrin to prothrombin leads to appearance of the thrombin-like activity of prothrombin towards S2238 chromogenic substrate. DesA and desABβ(15-42)2 fibrin forms did not cause any activation of prothrombin. From this observation we could suggested that amino acid residues of the 15-42 fragment of BβN-domain presented in desAB fibrin, cleaved in...

The relationship between platelet release and fibrinopeptide A cleavage from fibrinogen to form fibrin I in vitro was examined in blood allowed to clot undisturbed or with gentle agitation. In undisturbed or agitated blood platelet release and fibrin I formation occurred simultaneously. When hirudin was added to undisturbed blood it prevented platelet release as well as fibrin I formation. In c...

Fibrin has recently been shown to activate platelets through the immunoglobulin receptor glycoprotein VI (GPVI). In the present study, we show that spreading of human platelets on fibrin is abolished in patients deficient in GPVI, confirming that fibrin activates human platelets through the immunoglobulin receptor. Using a series of proteolytic fragments, we show that D-dimer, but not the E fra...

Fibrinogen Paris I, a congenital fibrinogen abnormality, is characterized by delayed fibrin aggregation and poor clot retraction owing to the replacement of normal gamma-chains by mutant gamma-chains, which are termed gamma-Paris I. Available evidence indicates that the structural abnormality involves the amino acid sequence near the COOH-terminus of the mutant chain and probably includes the r...

Plasma fibronectin has been shown to increase the binding of fibrin monomer to macrophages in vitro. In the present study we began characterization of the mechanism underlying this fibronectin activity. Fragments of fibronectin containing the amino terminus enhanced macrophage fibrin binding to the same extent as intact fibronectin on an equimolar basis. However, fibronectin fragments containin...

1. The time sequence of glomerular fibrin deposition, renal cortical fibrinolytic response and thrombocytopenia after thromboplastin infusion in rats has been established. 2. Fibrin clearance is rapid and is associated with markedly increased cortical fibrinolytic activity. 3. Plasma fibrinolytic activity was unchanged. 4. Experiments in which the dose-response relationship between thromboplast...

Sections of human tumors have been stained for fibrin deposits by the fluorescein label technic. Large clots of extravascular fibrin were demonstrated in most of the tumors tested. No such large clots were found in the several normal tissues investi gated. However, there was some staining of certain areas of connective tissue in both normal and tumor tissues. The fibrin deposition observed in t...

Our long-term research goal is to understand and exploit the natural biochemical properties of the fibrinogen/fibrin system for greater control of fibrin polymerization and degradation. In R21EB008463, we hypothesized that a fibrin knob fusion with thermo-responsive elastin-like peptide repeats will bind to fibrinogen polymerization pockets and prevent polymerization in a temperature-dependent ...

Sections of human tumors have been stained for fibrin deposits by the fluorescein label technic. Large clots of extravascular fibrin were demonstrated in most of the tumors tested. No such large clots were found in the several normal tissues investi gated. However, there was some staining of certain areas of connective tissue in both normal and tumor tissues. The fibrin deposition observed in t...