نتایج جستجو برای: fed batch process
تعداد نتایج: 1406141 فیلتر نتایج به سال:
This case study focuses on the scale-up of a Sp2/0 mouse myeloma cell line based fed-batch bioreactor process, from the initial 3-L bench scale to the 2,500-L scale. A stepwise scale-up strategy that involved several intermediate steps in increasing the bioreactor volume was adopted to minimize the risks associated with scale-up processes. Careful selection of several available mixing models fr...
This report discusses control of dissolved oxygen in a bioreactor where the oxygen supply is manipulated using the stirrer speed. In batch and fed-batch cultivations the operating conditions change significantly which may cause tuning problems. Analysis using a linearized process model shows that the process dynamics is mainly affected by changes in the volumetric oxygen transfer coefficient KL...
BACKGROUND Bioprocess development of recombinant proteins is time consuming and laborious as many factors influence the accumulation of the product in the soluble and active form. Currently, in most cases the developmental line is characterised by a screening stage which is performed under batch conditions followed by the development of the fed-batch process. Performing the screening already un...
Rhamnolipids find broad applications as natural surfactants, emulsifiers, and antibiotics because of their low toxicity, high biodegradability and environmental soundness. In this study, a pH stage-controlled process of fermentation of rhamnolipids by Pseudomonas aeruginosa O-2-2 was established. A yield of 24.06 g/L in batch fermentation was achieved in a 5-L fermentor via the optimization of ...
A process was developed for production of a candidate vaccine antigen, recombinant C-terminal heavy chain fragment of the botulinum neurotoxin serotype E, rBoNTE(Hc) inPichia pastoris.P. pastoris strain GS115 was transformed with the rBoNTE(Hc) gene inserted into pHILD4 Escherichia coli—P. pastoris shuttle plasmid. The clone was characterized for genetic stability, copy number, and BoNTE(Hc) se...
Astaxanthin is a main carotenoid pigment. Fed-batch fermentation bioprocesses of astaxanthin in 2.0 M fermentor have been investigated. Moreover, pH shift control was employed in fed-batch fermentation at 2.0 M scale. The results of astaxanthin fermentation showed that the fed-batch fermentation with pH shift strategy was the best among all the experiments, and the maximum astaxanthin concentra...
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