the batch fermentation production of human growth hormone (hgh) was studied in a newrecombinant escherichia coli. the effect of different levels of dissolved oxygen and glucoseconcentration on cell density and substrate utilization were evaluated. the results indicated that theoptimum level of dissolved oxygen was 25% of air saturation. the specific growth rate reached amaximum for an optimal g...

The object of research is a production of antibiotics by fed batch fermentation. An intelligent system with a structure of neural network and a genetic algorithm are used in nonparametric modeling of the fermentation process. The goal is to build a model for the prediction of fermentation eeciency. A priori knowledge of experts, who are controlling the production of antibiotics in industry, is ...

BACKGROUND 2,3-Butanediol (2,3-BDO) is a promising bio-based chemical because of its wide industrial applications. Previous studies on microbial production of 2,3-BDO has focused on sugar fermentation. Alternatively, biodiesel-derived crude glycerol can be used as a cheap resource for 2,3-BDO production; however, a considerable formation of 1,3-propanediol (1,3-PDO) and low concentration, produ...

BACKGROUND Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expressed using highly enriched media in shake flask cultures, which are generally not amenable to sca...

Superoxide dismutase (SOD) catalyzes the dismutation of the biologically toxic superoxide anion into oxygen and hydrogen peroxide and is deployed by the immune system to kill invading microorganisms. Extracellular SOD (EC-SOD) is a copper- and zinc-containing glycoprotein found predominantly in the soluble extracellular compartment that consists of approximately 30-kDa subunits. Here, we purifi...

Surfactin produced by B.subtilis BDCC-TUSA-3 from Maldex-15 as a growth associated product in conventional batch process. Maldex-15 is a cheap industrial by-product recovered during manufacturing of high fructose syrup from corn starch. Surfactin production was greatly improved in exponential fed-batch fermentation. Maldex-15 and other nutrients were exponentially fed into the culture based on ...

The integration of batch fermentation and membrane-based pervaporation process in a membrane bioreactor (MBR) was studied to enhance bioethanol production compared to conventional batch fermentation operated at optimum condition. For this purpose, a laboratory-scale MBR system was designed and fabricated. Dense hydrophobic Polydimethylsiloxane (PDMS) membrane was used for pervaporation. For fer...

The aim of this work was to develop a production process for the enzyme xyloglucan endo-transglycosylase from Populus tremula x tremuloides (PttXET16-34). The natural transglycosylating activity of this enzyme has previously been employed in a XETTechnology. This chemo enzymatic method is useful for biomimetic modification of cellulose surfaces and holds great potential for industrial applicati...

A process was developed for production of a candidate vaccine antigen, recombinant C-terminal heavy chain fragment of the botulinum neurotoxin serotype E, rBoNTE(Hc) inPichia pastoris.P. pastoris strain GS115 was transformed with the rBoNTE(Hc) gene inserted into pHILD4 Escherichia coli—P. pastoris shuttle plasmid. The clone was characterized for genetic stability, copy number, and BoNTE(Hc) se...

Paja Brava is a native South American grass with a high carbohydrate content. In the current work, the potential of using this feedstock for ethanol production using a simultaneous saccharification and fermentation (SSF) process with the xylose-fermenting yeast Pichia stipitis (Scheffersomyces stipitis) CBS6054 was investigated. The straw material was subjected to SO2 catalyzed steam pretreatme...