AIM To study the phenomenon that human erythroid leukemia K-562 cells are more sensitive to cytotoxic effect of antimetabolites when cultured in a serum-free medium than in a conventional medium containing fetal calf serum (FCS). METHODS Cytotoxic effects of methotrexate, azaserine and 5-fluorouracil were estimated by accessing the lactate dehydrogenase (LDH) activity of viable tumor cells. P...

Natural anionic polysaccharides fucosylated chondroitin sulfates (FCS) from sea cucumbers attract great attention nowadays due to their ability to influence various biological processes, such as blood coagulation, thrombosis, angiogenesis, inflammation, bacterial and viral adhesion. To determine pharmacophore fragments in FCS we have started systematic synthesis of oligosaccharides with well-de...

This paper proposes a fuzzy classifier system (FCS) using fuzzy rules given by hyper-cone membership functions. The hyper-cone membership function is expressed by a kind of radial basis function, and its fuzzy rules can be flexibly located in input and output spaces. Therefore, The FCS can generate excellent rules which have the best location and shape of membership functions. We apply the FCS ...

We describe CP-Rota, a new constraint programming application for rotating workforce scheduling that is currently being developed at our institute to solve real-life problems from industry. It is intended to complement FCS, a previously developed application. The advantages of CP-Rota over FCS are a significantly smaller and more maintainable code base, portability across a range of different l...

Almost every person has to deal with transgressions committed by a romantic partner and faces their negative psychological outcomes, coping strategies might be key understanding post-transgression dynamics forgiveness. We tested the construct validity of Inventory Strategies for Coping Partner's Transgression which include emotion (E-FCS), problem (P-FCS), meaning-focused (M-FCS) scales. Result...

Discrete-time survival analysis (DTSA) models are a popular way of modeling events in the social sciences. However, discrete-time data is challenged by missing one or more covariates. Negative consequences covariate include efficiency losses and possible bias. A approach to circumventing these multiple imputation (MI). In MI, it crucial outcome information models. As there little guidance on ho...

Reinforcement of movement is an important mechanism by which sensory feedback contributes to motor control for walking. We investigate how sensory signals from movement and load sensors interact in controlling the motor output of the stick insect femur-tibia (FT-) joint. In stick insects, flexion signals from the femoral chordotonal organ (fCO) at the FTjoint and load signals from the femoral c...

MOTIVATION Fluorescence Correlation Spectroscopy (FCS) is a popular tool for measuring molecular mobility and how mobility relates to molecular interaction dynamics and bioactivity in living cells. The FCS technique has been significantly advanced by its combination with super-resolution STED microscopy (STED-FCS). Specifically, the use of gated detection has shown great potential for enhancing...

A proliferative response of smooth muscle cells to activation of extracellular signal regulated kinases 1 and 2 (ERK1/2) has been linked to cardiovascular disease. In fully differentiated smooth muscle, however, ERK1/2 activation can also regulate contraction. Here, we use A7r5 smooth muscle cells, stimulated with 12-deoxyphorbol 13-isobutylate 20-acetate (DPBA) to induce cytoskeletal remodelin...