This paper presents how the function-behaviour-structure (FBS) ontology can be used to represent processes despite its original focus on representing objects. The FBS ontology provides a uniform framework for classifying processes and includes higher-level semantics in their representation. We show that this ontology supports a situated view of processes based on a model of three interacting wo...

BACKGROUND Fetal bovine serum (FBS) is the most widely used serum supplement for mammalian cell culture. It supports cell growth by providing nutrients, growth signals, and protection from stress. Attempts to develop serum-free media that support cell expansion to the same extent as serum-supplemented media have not yet succeeded, suggesting that FBS contains one or more as-yet-undefined growth...

Chinese Kunming mice (Mus musculus Km), widely used as laboratory animals throughout China, remain very refractory for embryonic stem (ES) cell isolation. The present study was aimed to evaluate the effects of hybridization with 129/Sv mice, and culture media containing fetal bovine serum (FBS) or Knockout serum replacement (KSR) on ES cell isolation from Kunming mice. The results demonstrated ...

FBS is a natural cocktail of most of the factors required for cell attachment, growth, and proliferation, effective for most types of human and animal (including insect) cells. Although in use for more than 50 years, FBS has never been fully characterized. Recent proteomic and metabolomic studies revelead approx. 1,800 proteins1,2 and more than 4,000 metabolites3 present in serum. However, the ...

Recently, a new approach of upstream detection of BVD virus in raw, unprocessed FBS has been described. This is a sandwich enzyme-linked immunosorbent assay (S-ELISA) designed to detect presence of BVD virus in the serum, using a commercially available diagnostic kit (Syracuse Bioanalytical, Syracuse, NY). In this study, the S-ELISA kit that uses raw (unprocessed) FBS as the testing sample was ...

Adult rat adrenal cells, infected with Kirsten murine sarcoma virus in early passage, transform consistently in 10% fetal bovine serum (FBS)-supplemented medium. Substitution of 3% horse serum (HS) for FBS reverses early foci and delays transformation. The influence of the serum on DNA synthesis, anchorage dependence, tumorigenicity, and subcellular Mr 21,000 transforming protein (p21) distribu...

We have studied the effects of recombinant hematopoietic growth factors, granulocyte-macrophage colony-stimulating factor (GM-CSF) and/or interleukin-3 (IL-3) on the globin program of adult human erythroid progenitors (BFUe) stimulated to terminal differentiation by erythropoietin under fetal bovine serum (FBS)-supplemented or FBS-deprived culture conditions. Fetal globin production by BFUe-der...

This paper presents how the function–behavior–structure (FBS) ontology can be used to represent processes despite its original focus on representing objects. The FBS ontology provides a uniform framework for classifying processes, and includes higher level semantics in their representation. We show that this ontology supports a situated view of processes based on a model of three interacting wo...