An improved pulsed-field electrophoresis program was developed to study differently sized chromosomes within the genus Saccharomyces. The number of chromosomes in the type strains was shown to be nine in Saccharomyces castellii and Saccharomyces dairenensis, 12 in Saccharomyces servazzii and Saccharomyces unisporus, 16 in Saccharomyces exiguus and seven in Saccharomyces kluyveri. The sizes of i...

To assess Borrelia burgdorferi sensu lato (the cause of Lyme borreliosis) seropositivity in Germany, we tested serum samples from health survey (2008-2011) participants. Seroprevalence was 5.8% among women and 13.0% among men; infection risk was highest among persons >60 years of age. Public health interventions, including education about risk factors and preventive measures, are needed.

with doxycycline (100 mg 2×/d) for 10 days; they clinically recovered, and laboratory abnormalities returned to reference ranges at discharge 12 days after admission. The remaining 22 persons did not report any malaise and were examined 1 month after tick bite. They appeared healthy at that time, and PCR and ELISA gave negative results, arguing against possible asymptomatic B. miya-motoi infect...

OBJECTIVE Western blotting (WB; immunoblotting) is a widely used tool for the serodiagnosis of Lyme borreliosis (LB), but so far, no generally accepted criteria for its performance and interpretation have been established in China. The present study was designed to determine the criteria for standardized Western blot for the predominant species of Borrelia burgdorferi sensu lato in China, in wh...

In Europe, 6 of the 11 genospecies of Borrelia burgdorferi sensu lato are prevalent in questing Ixodes ricinus ticks. In most parts of Central Europe, B. afzelii, B. garinii, and B. valaisiana are the most frequent species, whereas B. burgdorferi sensu stricto, B. bissettii, and B. lusitaniae are rare. Previously, it has been shown that B. afzelii is associated with European rodents. Therefore,...

To determine how often Slovenian children with acute peripheral facial palsy are infected with Borrelia burgdorferi sensu lato, 52 patients with peripheral facial palsy were included in this prospective clinical study. According to case definitions, the diagnosis of Lyme borreliosis was established in 56% of those patients. The diagnosis was confirmed in 41%, probable in 28%, and possible in 31...