The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills)...

lycopene is a natural and dominant pigment in tomato with coloring and antioxidant properties, which nowadays the demands for its extraction enhancement from tomato and tomato products are increasing. in this study, the effect of pectinase enzyme pre-treatment at 2- 10 ml/kg and also washing with 94% ethanol at 60°c for 5 seconds was studied. in these conditions, treating with 10 ml/kg enzyme c...

Alkaline pre-extraction and hydrothermal hydrolysis were carried out with eucalyptus chips. Two methods were used to determine and calculate the extraction yield of five-carbon sugars. One is an indirect method, based on the difference of pentosan content between the chips before and after pre-extraction. The other method is to directly measure the content of five-carbon sugars in the extractin...

Mulberry is one of the most widely used traditional Chinese medicines. Anthocyanins are the main bioactive components of mulberry, and possess important biological activities, such as antimicrobial, anti-inflammatory and antioxidant activities. This study investigated the ultrasound-assisted extraction (UAE) of anthocyanins from mulberry by using response surface methodology (RSM). The extracti...

This work reports a novel approach where three phase partitioning (TPP) was combined with microwave for extraction of mangiferin from leaves of Mangifera indica. Soxhlet extraction was used as reference method, which yielded 57 mg/g in 5 h. Under optimal conditions such as microwave irradiation time 5 min, ammonium sulphate concentration 40% w/v, power 272 W, solute to solvent ratio 1:20, slurr...

This work is focused on the chemical characterization of Maytenus ilicifolia extracts obtained from high-pressure CO2 extraction. The experiments were performed in a laboratoryscale unit, where the effect of particle size (35-200mesh), CO2 mass flow rate (1 to 3gmin), temperature (293 to 323K), pressure (100 to 250bar), and extraction time was investigated in terms of liquid yield and chemical ...

BACKGROUND One source of deoxyribonucleic acid (DNA) for genetic studies is the utilization of dried blood spots stored on paper cards (Guthrie cards) collected shortly after birth. These cards represent an important source of material for epidemiologic and population-based genetic studies. Extraction of DNA from these cards can lead to variable amounts of recovered DNA. We report here results ...

A method for stably purifying a functional dye, phycocyanin from Spirulina platensis was developed by a hexane extraction process combined with high pressure. This was necessary because this dye is known to be very unstable during normal extraction processes. The purification yield of this method was estimated as 10.2%, whose value is 3%-5% higher than is the case from another conventional sep...

This work was aimed to extract and characterise the essential oils presented in the leaves of Moringa Oleifera using supercritical fluid extraction (SFE) and compare the results against those using the conventional Soxhlet extraction method. The oils extracted were analysed using GC-MS. The compounds were identified according to their retention indices and mass spectra (EI, 70eV). The effects o...