conclusions based on this similarity and our bioinformatics evaluations, this mentioned alpha-amylase gene can be expressed in s. cerevisiae as extracellular enzyme. results the presence of alpha-amylase gene in recombinant bacteria was certificated by colony-pcr method. after extraction of recombinant vector from e. coli, the competent s. cerevisiae cells were transformed using polyethylene gl...

Pectinases are most significant enzymes of commercial sector. Among all the commercially produced industrial enzymes, pectinases occupy 25% share in overall enzyme market. have wide applications various processes such as fruit juice clarification, wastewater remediation and textile paper industry etc. Pectinase is suitable environment friendly tool nature for sustainable development. Although, ...

The aim of this study is to characterize tumor cell specific expression purinergic ecto-enzymes CD39 and CD73, associate prognostic significance these patterns in colorectal cancer (CRC) patients. Protein gene the target genes various CRC lines were assessed via Western Blot (WB) analysis Real Time PCR (RT-PCR). Additionally, vs stromal was analyzed from publicly available patient datasets. Fin...

In this study, Enterococcus faecalis proteolytic strains which have the potential to degradation of bovine milk proteins were isolated from Turkish White Pickled Cheeses and samples. E. found strong caseinolytic activity. The extracellular protease enzymes produced by 60 different samples analyzed in pattern bands on a stained SDS-PAGE gel. highest activity isolates determined at pH 7.0 40 ℃ fo...

Endophytic fungi inside a plant can degrade portion of lignin and cellulose. Penicillium is one the industrial microorganisms with advantage producing enzymes complete enzyme system that be secreted into extracellular space. The natural evolution ancient tree species from special geographic environments to screen out cellulase-producing strains excellent characteristics provides promising direc...

This study investigates the degradation characteristics, bacterial community structure, and mechanism of rice straw under different levels nitrogen (N) application depths return 1-mature-winter tillage in paddy fields a cold zone from perspective biodegradation by using nylon mesh bag situ culture method. Paludibacteraceae Ruminococcaceae were dominant bacteria process, their abundance decrease...

The synthesis of extracellular enzymes and extracellular polysaccharide (EPS) in Xanthomonas campestris pathovar campestris (Xcc) is subject to co-ordinate regulation by a cluster of genes called rpf (for regulation of pathogenicity factors). These genes are located within a 21.9 kb region of the chromosome isolated as the cosmid clone pIJ3020. The genes in the left-hand section of this region ...