A quantitative autoradiographic method for the determination of local rates of protein synthesis in brain in vivo is being developed. The method employs L-[1-14C]leucine as the radiolabeled tracer. A comprehensive model has been designed that takes into account intracellular and extracellular spaces, intracellular compartmentation of leucine, and the possibility of recycling of unlabeled leucin...

Adipose stromal cells are the primary source of local oestrogens in adipose tissue, aberrant production of which promotes oestrogen receptor-positive breast cancer. Here we show that extracellular matrix compliance and cell contractility are two opposing determinants for oestrogen output of adipose stromal cells. Using synthetic extracellular matrix and elastomeric micropost arrays with tunable...

The extracellular matrix represents a complex alloy of variable members of diverse protein families defining structural integrity and various physiological functions. The most abundant family is the collagens with more than 20 different collagen types identified so far. Collagens are centrally involved in the formation of fibrillar and microfibrillar networks of the extracellular matrix, baseme...

This study was performed to determine whether extracellular silver nanoparticles (AgNPs) production is a genus-wide phenotype associated with all the members of genus Morganella, or only Morganella morganii RP-42 isolate is able to synthesize extracellular Ag nanoparticles. To undertake this study, all the available Morganella isolates were exposed to Ag+ ions, and the obtained nanoproducts wer...

Abscission zone (AZ) development and the progression of abscission (detachment of plant organs) have been roughly separated into four stages: first, AZ differentiation; second, competence to respond to abscission signals; third, activation of abscission; and fourth, formation of a protective layer and post-abscission trans-differentiation. Stage three, activation of abscission, is when changes ...

Microbial electrochemical technologies are becoming an interest for the community due to their possible applications in wastewater treatment, biosensing, biosynthesis, and bioenergy. Fundamental these is use of electroactive microorganisms as bioelectrocatalysts. Recent studies have aimed elucidate electron transfer strategies microorganisms, with a keen extremophilic bacteria enhanced survivab...

Ovulation may constitute a cyclic, inflammatory-like process, wherein the increased expression of interleukin (IL)-1 and the biosynthesis of prostaglandins may be established corollaries. In this communication we hypothesize that glucocorticoids, potent anti-inflammatory principles, may exert an antiovulatory effect by interfering with ovarian IL-1-driven prostaglandin biosynthesis. To test thi...

objective(s): chitosan nanoparticles (cnps) were prepared based on the ionic gelation of chitoan with anionic compounds of ocimum basilicum leaf extract. materials and methods: after addition of ocimum basilicum leaf extract to chitosan solution, the physicochemical properties of the nanoparticles were determined by field emission scanning electron microscope (fesem), fourier transform infrared...

The ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) obtain energy or chemicals from pure substances residual substrates. Here, a new pyoverdine-producing Pseudomonas aeruginosa strain was isolated an MFC biofilm oxidizing glycerol, by-product biodiesel production. Strain EL14 investigated assess its electrogenic and...

The dark-detached leaves are widely used as a model to investigate senescence-delaying mechanisms of cytokinin (CК). role CK during senescence detached Arabidopsis in the light is less examined, although they provide unique opportunities for study interaction and photosynthetic apparatus. Within first four days incubation on water photosynthesis activity, chloroplast gene expression chlorophyll...