The transcriptional responses of drug treatments combined with a protein target prediction algorithm was utilised to associate compounds to biological genomic space. This enabled us to predict efficacy of compounds in cMap and LINCS against 181 databases of diseases extracted from GEO. 18/30 of top drugs predicted for leukemia (e.g. Leflunomide and Etoposide) and breast cancer (e.g. Tamoxifen a...

background: in gene therapy, the use of rna molecules as therapeutic agents has shown advantages over plasmid dna, including higher levels of safety. however, transient nature of rna has been a major obstacle in application of rna in gene therapy. methods: here, we used the internal ribosomal entry site of encephalomyocarditis virus and the 3’ non-translated region of poliovirus to design an en...

efg1 transcription factor is believed to be the main regulator of hyphal formation under many different conditions. in addition, it is responsible for positive regulation of the expression of several hyphal-specific genes. sap5, which encodes secreted aspartic proteinase, is one of the mentioned genes and is crucial for pathogenicity properties. in the present work we have established the exper...

human interferon β (inf-β) is a member of cytokines family which different studies have shown its immunomodulatory and antiviral activities. in this study an expression vector was designed and constructed for expression of human inf-β-1b either in shake flasks and bench top bioreactor. the designed vector was constructed based upon pet-25b(+) with t7 promoter. recombinant human beta interferon ...

Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

vicia sativa l., an annual winter growing leguminous plant, is a valuable source of protein and minerals for cattle. drought is one of the key stress factors that influence plant growth and development. in order to investigate common vetch physiological and molecular responses under normal irrigation (n) and two levels of drought stress [s1= 30% and s2= 10% field capacity (fc)], a greenhouse exp...

Background: DNA microarray is a useful technology that simultaneously assesses the expression of thousands of genes. It can be utilized for the detection of cancer types and cancer biomarkers. This study aimed to predict blood cancer using leukemia gene expression data and a robust ℓ2,p-norm sparsity-based gene selection method. Materials and Methods: In this descriptive study, the microarray ...

background: receptor for advanced glycation end products (rage) plays a causative role in diabetes. garlic (allium sativum) belongs to compounds with anti-glycation activity that can be considered as probable therapeutic approaches in delaying or preventing the onset of diabetes complications. the aim of this study was to evaluate the influence of garlic on the rage expression and proinflammato...

Aberrant DNA methylation has been reported as an important phenotype in acute myeloid leukemia. However the clinical significance of methylation changes has not been clear yet. In this study methylation Specific Melting Curve Analysis (MS-MCA) and real time PCR was used to assess the CDKN2B promoter hyper-methylation and gene expression in 59 Iranian acute myeloid leukemia (AML) patients. The i...

Background & objective: Microarray and next generation sequencing (NGS) data are the important sources to find helpful molecular patterns. Also, the great number of gene expression data increases the challenge of how to identify the biomarkers associated with cancer. The random forest (RF) is used to effectively analyze the problems of large-p and smal...