background: staphylococcus aureus can cause infections with a wide spectrum of illnesses ranging from benign skin infections to bloodstream infection leading to mortality. antimicrobial resistance especially methicillin resistance in s. aureus (mrsa strains) is currently problematic. the emergence of mrsa infections has developed in both the healthcare and the community settings.  the aim of th...

The alcohol dehydrogenase I (Adh1) gene of maize (Zea mays L.) was employed as a source of transcriptional, posttranscriptional, and translational regulatory sequences in the construction of an expression vector. By transforming the translation-initiating ATG and an ATG three triplets upstream from the translational termination triplet into NcoI sites (5'-CCATGG-3'), the maize Adh1 gene was con...

In this paper, the construction and evaluation of a chromosomal expression platform (CEP), which allows controlled gene expression following ectopic integration into the chromosome of Streptococcus pneumoniae, is described. CEP is based on the well-studied maltosaccharide-inducible system. To facilitate integration at CEP, a plasmid, pCEP, capable of replication in Escherichia coli, but not in ...

Abstract The objective of this work was to develop a method create and validate CRISPR-Cas systems different gRNAs in soybean (Glycine max) embryos. Two model genes were used for simple mutation with one gRNA or partial gene deletion two guides. inserted into the CRISPR transformation vectors by type IIS restriction enzyme subcloning inserting promoter + gRNA2 final vector using classic cloning...

Introduction Targeted integration (TI) allows fast and reproducible genetic modification of well characterized previously tagged host cells thus generating producer cells with predictable qualities. Concurrently, timelines are cut by 50% compared to random integration (RI) based cell line development. In contrast to commonly low productivities of cell lines generated by TI, we developed a syste...