The bacterial lactose converting enzyme beta-galactosidase (β-gal) and its gene (LacZ) have been studied for many years (1 and references therein) and are among the most utilized tools in molecular biology. The LacZ product, a polypeptide of 1029 amino acids, gives rise to the functional enzyme after tetramerization (2 and references therein) and is easily detected by chromogenic substrates eit...

We studied the human lysosomal alpha-mannosidase (MANB) by expressing the putative cDNA in mammalian cells, using the eucaryotic expression vector pCDE. The construct pCDE-MANB and pSV2-Neo were cotransfected into human alpha-mannosidase deficient fibroblasts and into a murine cell line and selected by culture in the presence of G418. Six G418 resistant 3T3 clones had increased alpha-mannosidas...

[This corrects the article on p. 2540 in vol. 62, PMID: 8779593.].

In the course of studies looking for differentially expressed genes, we isolated as a control a cDNA hybridizing to a highly and constantly expressed RNA. Further characterization of the hamster and human full-length cDNAs showed that they correspond to ribosomal protein S26 RNA. Sequence comparison revealed a very high degree of conservation at the proteic level amongst distantly related eukar...