Sex differentiation in fish is a highly labile process easily reversed by the use of exogenous hormonal treatment and has led to environmental concerns since low doses of estrogenic molecules can adversely impact fish reproduction. The goal of this study was to identify pathways altered by treatment with ethynylestradiol (EE2) in developing fish and to find new target genes to be tested further...

In this study the effect of Centella asiatica L. extract was studied against genotoxic doses of ethinylestradiol on human lymphocytes culture. The different doses of C. asiatica L. extract i.e. 1.075x10-4, 2.125x10-4 and 3.15x10-4 g/ml were treated separately with 5 μM of ethinylestradiol. Similar treatments were given with 10 μM of ethinylestradiol. The treatments result in a significant dose ...

In the present study the effect of Centella asiatica L. extract was studied against the genotoxic doses of ethinylestradiol on human lymphocytes culture. The different doses of C. asiatica L. extract i.e. 1.075×10 -4 , 2.125×10 -4 and 3.15×10 -4 g/ml were treated separately with 5 μM of ethinylestradiol. Similar treatments were given with 10 μM of ethinylestradiol. The treatments result in a si...

Adult ovariectomized Sprague-Dawley rats were administered a single initiating dose of 200 mg diethylnitrosamine (DEN)/kg, i.p. 17a-ethyn\ U-sirailiol (!•'.!•'...) was then chronically administered to the rats by means of s.c. Silastic implants at an estimated dose of 90 MgEE2/kg/day. Hepatic •Y-glutamyltranspeptidase-positivefoci were evaluated after 20-60 (+20,+30,...,+60) weeks of chro...

This study aims to characterize the estrogen receptor (er) in sand goby (Pomatoschistus minutus) and determine the temporal effects of 17α-ethinyl estradiol (EE2) on erα and vitellogenin (vtg) gene expression in males. Two partial cDNA sequences (erα and erβ1) are presented showing conserved structural features with ers of other species. Transcript levels for both ers were low in control fish b...

This chapter describes the scientific evidence and regulatory debates concerning one of these oestrogens, ethinyloestradiol (EE2), an active ingredient in the birth control pill. First developed in 1938, it is released to the aquatic environment via wastewater treatment plants. Although it is now clear that wildlife species are exposed to and impacted by a cocktail of endocrine disrupting chemi...

Industrial and domestic sewage effluents have been found to cause reproductive disorders in wild fish, often as a result of the interference of compounds in the effluents with the endocrine system. This thesis describes laboratory-based exposure experiments and a field survey that were conducted with juveniles of the three-spined stickleback, Gasterosteus aculeatus. This small teleost is a comm...

Most studies of hormonal activity in rivers have focused on inputs from sewage treatment works (STW), and their consequences for endocrine disruption in fish. It is possible that livestock is contributing to this hormonal activity in rivers. This study represents a search for evidence of steroid hormone contamination in streams associated with livestock farms. The majority of the 10 sites selec...

UV/H₂O₂, which is an advanced treatment technology used to reduce multiple contaminants, is effective in potable water treatment. Simultaneous degradation effects and kinetics of three types of coexisting micropollutant estrogens (steroid estrogens, SEs), including estrone (E1), 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2), in deionized water were studied. Experiments were carried out wit...