Background The sensitivity of smear microscopy for diagnosis of tuberculosis might be improved through treatment of sputum with sodium hypochlorite and application of fluorescent microscopy. This study aimed to determine the agreement between direct Fluorescent Microscopy and Ziehl-Neelsen concentration technique by their ability of detecting acid fast bacilli in resource poor settings. Metho...

Negative staining technique of Heine is an easy, inexpensive, and rapid way of screening for coccidian parasites of the intestinal tract. But its use as a routine technique for screening of Cryptosporidium, Cyclospora, and Cystoisospora is restricted due to its sensitivity being lower than the gold standard method of modified Ziehl-Neelsen staining. This paper emphasises the modification of ori...

Cryptosporidium felis and C. meleagridis in Persons with HIV, Portugal To the Editor: Cryptosporidium, a pathogenic protozoan parasite with a worldwide distribution, causes diar-rheal illness in humans and animals. The parasite can be transmitted from human to human through fecal-oral contact (household contact and noso-comial transmission), sexual contact, ingestion of contaminated food or wat...

The methanol extracts of leaves of Bauhinia acuminata (MESF) and their different fractions obtained from modified Kupchan partitioning method i.e. methanol, pet ether, carbon tetrachloride, chloroform, and aqueous soluble fractions were subjected to biological screening such as total phenolic content and antioxidant activity screening.The amount of total phenolic content differed in di...

background: tween as a substitute for diethyl ether in the formalin-ether sedimentation technique was evaluated for para­site detection. methods: fresh fecal material free of parasites with 10% formalin to prepare standardized specimen was thoroughly pooled. this specimen was divided into 5 equal portions; one was without infection, and each of the others was individually seeded with entamoeba ...