Estradici and progesterone receptor concentrations were determined in cytoplasmic and nuclear fractions of endometrial cancer specimens taken from 43 postmenopausal women. In addition to receptors, ornithine decarboxylase and 17/?-estradiol hydroxysteroid dehydrogenase activities were also meas ured before and after antiestrogen treatment to evaluate hor mone sensitivity of the tumors. Fifteen ...

BACKGROUND Prolactin is a peptide hormone necessary for normal breast development that may contribute to breast tumorigenesis. Estrogen is a significant positive regulator of prolactin synthesis; therefore, raloxifene, a selective estrogen receptor modulator under study as a breast cancer prevention agent, may modulate both estradiol and prolactin levels by inhibiting estradiol from binding to ...

This is the first report on estrogen-dependent growth of human-derived colon carcinoma cells. Under selected conditions, growth of suhconfluent Caco-2 cells is triggered by estradiol. Cell growth is estradiol concentra tion dependent, with maximal effect occurring ut uhout 0.4 n\i. ( i row lit is prevented by two différentantiestrogens: the partial agonist, OH-Tamoxifen, and the pure antagonis...

Abstract Background and Aims Female rats excrete more urine sodium than male with similar blood pressure (BP). However, it is not clear whether the sex difference in natriuresis conserved menopausal rats. We assessed natriuretic response renal transporter activity ovariectomized female infused angiotensin II (ANGII) could be affected by estrogen supplementation. Method Six-week-old Sprague-Dawl...

objective(s): neuroprotective effects of female gonadal steroids are mediated through several pathways involving multiple peptides and receptors after traumatic brain injury (tbi). two of these peptides are including the regulatory peptides neuromedin u (nmu) and neuromedin s (nms), and their common receptor neuromedin u2 receptor (nmur2). this study investigates the effects of physiological do...

The cytoplasmic 4 S receptor protein that specifically binds the steroid hormone 17/3-estradiol has been studied in soluble extracts of rat uterine tissue at physiological ionic strengths. After labeling the protein with [aH]estradiol, two related interactions of the receptor with other macromolecular components have been detected and characterized by DNA-cellulose chromatography and sucrose gr...

Gonadotropin-releasing hormone (GnRH) neurons are critical to controlling fertility. In vivo, estradiol can inhibit or stimulate GnRH release depending on concentration and physiological state. We examined rapid, nongenomic effects of estradiol. Whole-cell recordings were made of GnRH neurons in brain slices from ovariectomized mice with ionotropic GABA and glutamate receptors blocked. Estradio...

Human breast cancer cells (MCF-7, maintained in long-term culture) contain separate estrogen receptors specific for either 17/î-estradiol or estrone. Utilizing optimum conditions for the protamine sulfate assay, it has been possible to demonstrate both receptors in the 0.6 M KCI extract of nuclei and in the cytosol. Similarly, in the exchange assay, high-affinity lowcapacity binding sites for ...

BACKGROUND The beneficial vasoprotective effects of a postmenopausal estrogen replacement therapy may be prevented by a concomitant administration of progestins. To investigate the differential effects of estrogens and progesterone, we examined their influence on AT(1) receptor gene expression in vascular smooth muscle cells (VSMCs). METHODS AND RESULTS 17beta-Estradiol caused downregulation ...

This study investigated pubertal changes in neural and behavioral responses to estradiol. Gonadectomized pre- and postpubertal male hamsters (Mesocricetus auratus) were treated with 0.00, 0.05, 0.10, or 0.25 mg estradiol and tested 1 week later for sexual behavior with a receptive female. Estradiol activated behavior in postpubertal, but not prepubertal, males. In contrast, estrogen receptor al...