Influence of duration of cholesterol feeding on esterification of fatty acids and hydrolysis of cholesteryl esters was studied in cell-free preparations of aorta from White Cameau pigeons. Esterification of fatty acids required ATP and CoA; greater than 80* of the esterifying activity was located in the particulate fraction obtained by centrifugation at 105,000 X g (after a preliminary centrifu...

We investigated effects of fluvastatin, a 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitor, and its major metabolites, M2 and M4, on CuSO4-induced low-density lipoprotein (LDL) oxidation and cholesteryl ester accumulation in mouse peritoneal macrophages. All the test compounds inhibited LDL oxidation, and M2 had the most potent effect comparable to vitamin E. When LDL was pre...

Immobilized Candida antarctica lipase B, Novozym 435, was used as the biocatalyst in the esterification of adipic acid with four different isomers of butanol (n-butanol, sec-butanol, iso-butanol, and tert-butanol). Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology approach with a four-factor-five-level central composite design concerning imp...

In recent publications (1, 2) we have shown that bile salts exert a marked influence on the proportion between the cholesterol fractions in incubated human and dog sera. In small concentrations all of the bile salts studied inhibit, increasingly with increasing amounts, the esterification of free cholesterol which occurs normally during incubation of both types of serum. In larger concentration...

Recently, biomass-derived energy has been receiving more and more attention due to the depletion of conventional fossil sources at a faster rate, as well as major environmental issues.1 Biodiesel obtained by transesterification of vegetable oils and animal fats provides an alternative fuel option for the future.2 Transesterification of oils and animal fats produce glycerol in large excess (10 k...

1. When [4-(14)C]cholesterol, attached to beta-globulin or dispersed with Tween 20, was incubated with fresh rabbit (New Zealand albino females) plasma, 30-47% esterification was observed. The optimum pH was 6.8. This esterification was accomplished by the transfer of fatty acids from the C-2 position of lecithin (phosphatidylcholine) to cholesterol. 2. There was no evidence that triglycerides ...