This study was aimed to evaluate the efficacy of dipstick tests (leukocyte esterase and nitrite) in diagnosis of spontaneous bacterial peritonitis (SBP) in cirrhotic patients. Forty six children with ascites hospitalized between 2009 and 2010 in Children Medical Center were enrolled in this study. Reagent strip assays for leukocyte esterase and nitrite were performed on ascetic fluid and the re...

This study aims to identify yeasts from spontaneously fermented herbs used for Van herby cheese and evaluate their enzymatic activities. Sequencing of partial 26S rRNA gene revealed the species Pichia membranifaciens (n = 8), Kazachstania servazzii 6), Debaryomyces hansenii 2), Kluyveromyces marxianus fermentans 2). Enzyme profiles were determined using API-ZYM strips. The isolates had diverse ...

We have developed an esterase inhibition test to investigate the effects of 10 toxicants, including six metals (cadmium, chromium, copper, lead, mercuric chloride, and titanium) and four organics (benzene, ethyl acetate, toluene, and vinyl acetate) in three species of the benthic rotifer genus Lecane (L. hamata, L. luna, and L. quadridentata). Metals affect esterase inhibition by an average val...

The studying of enzymatic esterification of carboxylic acids and higher alcohols was performed by esterase Saccharomyces cerevisiae in water-organic medium. Investigation of the enzyme specificity to acetic substrates showed the best result with acetic acid in esterification reactions with ethanol whereas within other carboxylic acids the esterification decreased with acids: hexanoic > pentanoi...

1. Clear, filtered glycerol extracts of chopped liver, spleen, and kidney contain an ester-splitting ferment. 2. The esterase content of extracts of the liver and spleen of normal dogs is reasonably constant. 3. The amount of esterase in the liver does not appear to vary to any great extent from the normal, in poisoning with chloroform and phosphorus. 4. Feeding large quantities of sugar and in...

Pepstatin is a potent peptidyl inhibitor of various malarial aspartic proteases, and also has parasiticidal activity. Activity of pepstatin against cultured Plasmodium falciparum is highly variable depending on the commercial source. Here we identify a minor contaminant (pepstatin butyl ester) as the active anti-parasitic principle. We synthesize a series of derivatives and characterize an anal...

A bacterial strain named A216, with feruloyl esterase producing capability, was isolated from forest soil after enrichment. The strain was identified at the species level by morphology, physiological-biochemical tests and 16S rRNA gene sequencing assay. The results revealed that strain A216 was accurately identified as Burkholderia fungorum, and the ITS sequence of the species was submitted to ...

Asian papaya fruit fly, Bactrocera papayae Drew & Hancock and carambola fruit fly, Bactrocera carambolae Drew & Hanckock (Diptera: Tephritidae) are the major agricultural pests, especially fruits and vegetables, in Malaysia. A vertical type polyacrylamide gel electrophoretic technique was used for the first time to compare the esterase isozyme banding patterns between the larvae of Bactrocera p...

Using image-analyzing equipment, we measured the effect of quinidine on the activity of naphthol AS-D chloroacetate esterase, alpha-naphthyl butyrate esterase, alpha-naphthyl acetate esterase, and acid phosphatase in individual cells of a human Hep G2 hepatoma cell line. The impact of the drug on the morphology of the cells was also observed. Depending on the concentration of quinidine applied,...

The present study evaluated the usefulness of the 1-min leukocyte esterase-nitrite tests in a tertiary-care hospital as a screening procedure to detect significant bacteriuria and correlated the findings with culture results. A total of 531 urine samples were reviewed, of which 484 were evaluated. Of the evaluated samples, 113 positive cultures (23.4%) were found, of which 93 (82.3%) were detec...