background and purpose: brain-derived neurotrophic factor (bdnf) is present in the hippocampus and cerebral cortex in high concentrations and has an important role in development, maintenance and regeneration of the nervous system. the aim of this study was to produce active bdnf in e.coli using transforming plasmid. methods: to construct an expression plasmid of bdnf, a gene coding for it was ...

results the results showed that the isolated e. coli strains were sensitive to these antibiotics: erythromycin (52.94%), tetracycline (76.47%), ceftazidime (41.17%), cefixime (35.29%), penicillin (76.47%), ampicillin (58.82%) and nalidixic acid (41.17%). examination of the herbal extracts showed that the highest maximum inhibitory concentration (mic) against drug resistant e. coli was 200 ppm. ...

background and objectives: bacteria need iron for growth and most of them can actively acquire fe ions using especial iron-chelating proteins which named siderophores. we aimed to determine the frequencies of iuca, iron and irp2 genes in the uropathogenic escherichia coli (upec) isolates. we also analyzed the effects of siderophore genes beside iron supplements on growth rate of the isolates. m...

background: the emergence and propagation of different phylogenetic groups of antimicrobial-resistant e. coli have become a worldwide health concern in human and veterinary medicine. therefore, the evaluation of the phylogenetic distribution of antibiotic-resistant e. coli is important for therapeutic and economic purposes. the aims of this study were to determine phylogenetic groups and patter...

background: the aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus pcr (eric-pcr) analysis of campylobacter spp. isolated from children with diarrhea in iran. methods: a total of 200 stool specimens were obtained from children under 5 years during july 2012 to july 2013. detection of c. jejuni and c. coli was perfo...

background: campylobacter jejune and c. coli are recognized as the most common bacteriological causes of gastroenteritis in humans. in this study identification of campylobacter jejuni and campylobacter coli from samples using pcr was explored. methods: detection was performed using diarrheal samples collected 8 from 117 children. the genomic dna of samples was extracted by phenol-chloroform me...

background and objectives: the therapeutic options for diseases caused by escherichia coli are limited. in this study we investigated the presence of virulence factors among enteropathogenic escherichia coli (epec) strains and their antibiotic resistance patterns. the isolates were also checked for the presence of class1 integrons and gene cassettes. materials and methods: this study included 7...

background and objectives: shiga toxin-producing escherichia coli ( stec) have emerged as human pathogens and contamination of foods of animal origin has been a major public health concern. the aim of the present study was to determine the dissemination of stec in healthy and diarrheic calves in urmia region which is located in west azerbaijan province, iran. materials and methods: in the curre...

conclusions the analysis recommended that ta genes are prevalent in clinical isolates of e. coli strains. the analysis revealed that hipba ta system is associated with biofilm formation. results the results of the analysis revealed that 107 e. coli isolates were potent for biofilm formation by cra. the findings by microtiter plates showed that 102 e. coli isolates were biofilm producers. the re...

conclusions the alcoholic extract of p. harmala was very effective against the selected esbl-producing e. coli isolates harboring the tem gene. therefore, it could be suggested as an antibacterial agent in the future. more researches are necessary for detecting the mechanism of this plant’s behavior and its pharmacological effects. results results of this study showed that in disk diffusion met...