نتایج جستجو برای: escs

تعداد نتایج: 1878  

Journal: :The International journal of developmental biology 2013
Debasree Dutta

Embryonic Stem Cells (ESCs) are derived from the inner cell mass of blastocysts. They have the unique potency to differentiate into diverse lineages. Hence, they are bestowed with the term pluripotency. Several mechanisms have been implicated in maintaining the pluripotency of ESCs. This review will focus on the role of signaling pathways in regulating ESC pluripotency among diverse mammalian s...

2017
Shiqi She Qucheng Wei Bo Kang Ying-Jie Wang

Embryonic stem cells (ESCs) have unlimited expansion potential and the ability to differentiate into all somatic cell types for regenerative medicine and disease model studies. Octamer‑binding transcription factor 4 (OCT4), encoded by the POU domain, class 5, transcription factor 1 gene, is a transcription factor vital for maintaining ESC pluripotency and somatic reprogramming. Many studies hav...

2017
Raghu Ram Edupuganti Arigela Harikumar Yair Aaronson Alva Biran Badi Sri Sailaja Malka Nissim-Rafinia Gajendra Kumar Azad Malkiel A. Cohen Jung Eun Park Chikdu S. Shivalila Styliani Markoulaki Siu Kwan Sze Rudolf Jaenisch Eran Meshorer

Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from t...

2017
Andrea Corsinotti Frederick Ck Wong Tülin Tatar Iwona Szczerbinska Florian Halbritter Douglas Colby Sabine Gogolok Raphaël Pantier Kirsten Liggat Elham S Mirfazeli Elisa Hall-Ponsele Nicholas P Mullin Valerie Wilson Ian Chambers

Deletion of Sox2 from mouse embryonic stem cells (ESCs) causes trophectodermal differentiation. While this can be prevented by enforced expression of the related SOXB1 proteins, SOX1 or SOX3, the roles of SOXB1 proteins in epiblast stem cell (EpiSC) pluripotency are unknown. Here, we show that Sox2 can be deleted from EpiSCs with impunity. This is due to a shift in the balance of SoxB1 expressi...

Journal: :Cell 2015
Bin Xia Yang Chadi A. EL Farran Hong Chao Guo Tao Yu Hai Tong Fang Hao Fei Wang Sharon Schlesinger Yu Fen Samantha Seah Germaine Yen Lin Goh Suat Peng Neo Yinghui Li Matthew C. Lorincz Vinay Tergaonkar Tit-Meng Lim Lingyi Chen Jayantha Gunaratne James J. Collins Stephen P. Goff George Q. Daley Hu Li Frederic A. Bard Yuin-Han Loh

Embryonic stem cells (ESCs) repress the expression of exogenous proviruses and endogenous retroviruses (ERVs). Here, we systematically dissected the cellular factors involved in provirus repression in embryonic carcinomas (ECs) and ESCs by a genome-wide siRNA screen. Histone chaperones (Chaf1a/b), sumoylation factors (Sumo2/Ube2i/Sae1/Uba2/Senp6), and chromatin modifiers (Trim28/Eset/Atf7ip) ar...

Journal: :World Journal of Biology Pharmacy and Health Sciences 2023

Mesenchymal stem cells (MSCs) are increasingly becoming the focal point of research in regenerative medicine due to their potential differentiate into several different lineages including those non-mesodermal origin. With reports successful differentiation MSCs cell types such as osteocytes, chondrocytes, cardiocytes and even fully functional neurons, it is more important than ever understand s...

Journal: :Stem cells 2009
Moorthy P Ponnusamy Shonali Deb Parama Dey Subhankar Chakraborty Satyanarayana Rachagani Shantibhusan Senapati Surinder K Batra

Embryonic stem cells (ESCs) maintain self-renewal while ensuring a rapid response to differentiation signals, but the exact mechanism of this process remains unknown. PD2 is the human homolog of the RNA polymerase II-associated factor 1 (Paf1). The Paf1/PD2 is a member of the human PAF complex that consists of four other subunits, hCdc73, hLeo1, hCtr9, and hSki8, and is involved in the regulati...

Journal: :PLoS ONE 2006
Qizhou Lian KengSuan Yeo Jianwen Que EileenKhiaWay Tan Fenggang Yu Yijun Yin Manuel Salto-Tellez Reida Menshawe El Oakley Sai-Kiang Lim

BACKGROUND Differentiation of embryonic stem cells (ESCs) into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage...

2012
Wen Cheng Lizao Chen Shengsheng Yang Jie Han Dongxia Zhai Jian Ni Chaoqin Yu Zailong Cai

BACKGROUND Puerarin is a major isoflavonoid compound extracted from Radix puerariae. It has a weak estrogenic action by binding to estrogen receptors (ERs). In our early clinical practice to treat endometriosis, a better therapeutic effect was achieved if the formula of traditional Chinese medicine included Radix puerariae. The genomic and non-genomic effects of puerarin were studied in our Lab...

2015
Lauren Walker Laura Baumgartner Kevin C. Keller Julia Ast Susanne Trettner Nicole I. zur Nieden

Many industrial chemicals and their respective by-products need to be comprehensively evaluated for toxicity using reliable and efficient assays. In terms of teratogenicity evaluations, the murine-based embryonic stem cell test (EST) offers a promising solution to screen for multiple tissue endpoints. However, use of a mouse model in the EST can yield only a limited understanding of human devel...

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