OBJECTIVE To determine the susceptibility pattern of extended spectrum ß-lactamase (ESBL) producing Gram negative isolates from various clinical specimens. STUDY DESIGN Descriptive study. PLACE AND DURATION OF STUDY Microbiology Department, Armed Forces Institue of Pathology, Rawalpindi, from January 2008 to January 2009. METHODOLOGY A total of 308 ESBL producing isolates from various cli...

Extended spectrum beta-lactamase (ESBL) production by Klebsiella sp. and E. coli is an emerging problem. In this study, 107 clinical isolates (53 E. coli, 47 K. pneumoniae and 7 K. oxytoca) screened as ESBL producers by the NCCLS disk diffusion procedure were submitted to a double disk confirmatory test (DDT) and to the E-test double strip for confirmation of ESBL production by demonstration of...

From a total of 320 bacterial samples from wound swab and urine 169 (53%) gram-negative bacteria were isolated, of which 42 (25%) extended-spectrum beta-lactamase (ESBL) producers were detected by double-disk synergy test. ESBL producers were significantly more resistant against amoxiclav, Co-trimoxazole, ciprofloxacin, amikacin and gentamicin than non-ESBL producers. Among the 42 ESBL producer...

PURPOSE Amp C beta-lactamase are Ambler class C enzymes that confer resistance to extended spectrum cephalosporins and are not inhibited by beta-lactamase inhibitors. Their detection is crucial, since the phenotypic tests are not standardised leading to ambiguity in interpretation of results. This study was done to detect the types of Amp C prevalent in Escherichia coli and Klebsiella pneumonia...

Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in high-risk environment companion animal clinic two patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs cats, hand staff 298 surfaces were for ESBL-E. Seventeen hospitalized patients (23%) ten (3%) ...

We assessed Escherichia coli ST131 and its H30 and H30-Rx subclones for virulence genes, antimicrobial resistance, and extended-spectrum beta-lactamase (ESBL) type. Although both subclones were associated with ESBL production, H30-Rx isolates had higher resistance scores and were associated specifically with CTX-M-15. Three virulence genes (iha, sat, and iutA) were more prevalent among H30 than...

Extended-spectrum beta-lactamase (ESBL)-producing pathogens are emerging as a cause of urinary tract infections (UTI) worldwide. In this matched-case control study, clinical characteristics and associated risk factors for ESBL UTI were evaluated. In a total of 463 positive urine cultures, 48 (10.4%) (from 39 patients, 23 boys) were phenotypically ESBL-producing bacteria. The most frequently iso...

Neurosarcoidosis affects 5%-10% of Sarcoidosis patients. The etiology of a sarcoidosis flare has been unknown. Several factors have been implicated in the pathogenesis of an acute flare, including genetic, environmental and infectious agents. We report the case of a 52-year-old female with known neurosarcoidosis who presented with altered sensorium on two occasions. On both episodes she had lep...

Corresponding Author: Viera Lovayová Department of Public Health and Hygiene, Faculty of Medicine, University of P.J. Safarik in Košice, Trieda SNP 1, 040 11 Košice, Slovakia Phone: +421915932031 Email: [email protected] Abstract: The beta-lactamases with extended spectrum of activity (ESBL) are medically one of the most important group of enzymes. The presented study provides identificati...

Introduction: Extended spectrum beta-lactamase (ESBL) producing Escherichia coli generate a major problem for clinical therapeutics and epidemiological study. The incidence of ESBL producing strains among clinical isolates has been steadily increasing during the past few years, and remains an important cause of failure of therapy with cephalosporins. The aim of this study was to determine the a...