BACKGROUND Mycobacterium tuberculosis (Mtb) infection may cause overt disease or remain latent. Interferon gamma release assays (IGRAs) detect Mtb infection, both latent infection and infection manifesting as overt disease, by measuring whole-blood interferon gamma (IFN-γ) responses to Mtb antigens such as early secreted antigenic target-6 (ESAT-6), culture filtrate protein 10 (CFP-10), and TB7...

background: discriminating latent tuberculosis infection (ltbi) from active tbi may be challenging. the objective of this study was to produce the recombinant l-alanine dehydrogenase (aladh) antigen and evaluate individuals with ltbi, those with active tbi, and uninfected individuals by enzyme-linked immunospot assay (elispot) in order to distinguish ltbi from active tbi. methods: this explorat...

The ESX-1 secretion system exports the immunomodulatory protein ESAT-6 and other proteins important in the pathogenesis of Mycobacterium tuberculosis. Components and substrates of ESX-1 are encoded at several loci, but the regulation of the encoding genes is only partially understood. In this study, we investigated the role of the MprAB two-component system in the regulation of ESX-1 activity. ...

Penyakit TBC yang disebabkan oleh bakteri Mycobacterium tuberculosis masih menjadi penyakit infeksi berbahaya hingga saat ini. Indonesia salah satu negara menyumbang angka kasus TB terbanyak di dunia. Vaksin BCG telah digunakan selama ini juga memiliki kelemahan dalam pencegahan TBC. Penelitian menyajikan desain vaksin secara in silico dirancang berdasarkan protein Rv1973, merupakan outer membr...

Mycobacterium tuberculosis 6-kDa early secretory antigenic target (ESAT-6) is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand (FL) that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid (pIRES-epitope-peptides-FL) encoding three T cell epitope...

BACKGROUND Although currently available IGRA have been reported to be promising markers for TB infection, they cannot distinguish active tuberculosis (TB) from latent infection (LTBI). OBJECTIVE Children with LTBI, active TB disease or uninfected were prospectively evaluated by an in-house ELISPOT assay in order to investigate possible immunological markers for a differential diagnosis betwee...