A highly efficient third generation catalyst, CaCu3Ti4O12 (CCTO) shows excellent photoelectrochemical (PEC) and photocatalytic ability. As only 4% part of the solar spectrum covers UV light, thus it is highly desirable to develop visible light active photocatalyst materials like CCTO for effective solar energy conversion. A direct band transition with a narrow band gap (1.5 eV) was observed. Un...

The ability of time-resolving enthalpy (∆H) and structural volume (∆V) changes in the nanoto μs time range offered by laser-induced optoacoustic spectroscopy (LIOAS) opens the possibility of a stepwise thermodynamic analysis of chromophore-medium interactions upon photoinduced reactions in biological systems. We applied LIOAS to biological photoreceptors, as well as to model systems, with the p...

Erythrosin B and trypan blue are tested and compared for their effectiveness as vital exclusion stains for mammalian cells in monolayer culture. Both stains are supposed to mark cells that have lost membrane integrity. Fluorescein diacetate (FDA), an efficient vital inclusion stain, is used as a control, as it marks cells retaining membrane integrity. Erythrosin B and FDA are used as fluorescen...

The role of the rotational dynamics of actin filaments in their interaction with myosin was studied by comparing the effect of myosin subfragment 1 (S1) with two other structural perturbations, which have substantial inhibitory effects on activation of myosin ATPase and in vitro motility of F-actin: (1) binding of the antibody fragment Fab(1-7) against the first seven N-terminal residues and (2...

We have studied the effect of gelsolin, a Ca-dependent actin-binding protein, on the microsecond rotational dynamics of actin filaments, using time-resolved phosphorescence (TPA) and absorption anisotropy (TAA) of erythrosin iodoacetamide attached to Cys374 on actin. Polymerization of actin in the presence of gelsolin resulted in substantial increases in the rate and amplitude of anisotropy dec...