The group G antigen of lactobacilli was identified as a negatively-charged cell wall polysaccharide. The components of the preparation isolated from cell walls of L. salivarius subsp. salivarius by mild acid hydrolysis were glucose, galactose and lesser amounts of rhamnose, N-acetylglucosamine and phosphate. Quantitative serological studies on acid-released polysaccharide and enzymic lysates of...

IT has long been known that the polysaccharide chitin when completely hydrolysed yields glucosamine and acetic acid; in recent years it has indeed been shown conclusively that the basic unit of the polysaccharide is N-acetylglucosamine. The evidence for the latter statement rests, firstly, on the discovery by Karrer & Hofmann [1929] ofan enzyme in Helixpomatiawhich is able to hydrolyse chitin t...

A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed t...

The ultramicro (1-microL samples) assay of serum or plasma triglycerides that we describe here is potentially applicable to 1-nL samples and to isolated cells. This technically simple method involves only three reactions: (a) enzymic hydrolysis with lipase and alpha-chymotrypsin; (b) conversion by glycerol kinase of the liberated glycerol and of adenosine triphosphate added in excess to glycero...

NAD+ glycohydrolase (NADase; EC 3.2.2.5) is an enzyme that catalyses hydrolysis of NAD+ to produce ADP-ribose and nicotinamide. Its physiological role and the regulation of its enzymic activity have not been fully elucidated. In the present study, the mechanism of self-inactivation of NADase by its substrate, NAD+, was investigated by using intact rabbit erythrocytes and purified NADase. Our re...

Achilli G, Cellerino GP, d'Eril GM, et al. 1995. Simultaneous determination of 27 phenols and herbicides in water by high-performance liquid chromatography with multi-electrode electrochemical detection. J Chromatogr 697:357-362. Ahmed N, Hale K. 1994. A microassay for urinary phenol using capillary gas chromatography and optimised enzymic hydrolysis. Clin Chim Acta 230:201-208. Amlacher E, Rud...

with 50 % (v/v) ethanol and radioactivity determined by liquid-scintillation counting. The separation procedure used is capable of separating cyclic AMP from all known naturally occurring adenine nucleotides. Results showed that the ‘total‘ membrane fraction from barley leaves possesses adenylate cyclase activity equivalent to 26pmol of cyclic AMP formed/min per mg of membrane protein. The lOOO...

We describe a sensitive, specific liquid-chromatographic determination of penbutolol and its 4-hydroxy metabolite in plasma and urine. The method involves a simple organic extraction, evaporation of the solvent, reconstitution in methanol/water, and injection into the chromatograph. Penbutolol, its metabolites, and the internal standard, propranolol, are resolved on a CN reversed-phase column a...