We describean automated assay for digoxinthat requiresa 2O0-L sample of serum. Total analysistime is 18 mm. The methodis extremely precise, withwithin-runCVs of 2.6, 1.6, and 4.9%, respectively, at 0.5, 1.5, and 3.5 j of digoxinper liter (n = 20). The lower limitof detectionis 0.2 g of digoxin per liter.For patients’samples, the correlationwith AlA (x) is excellent(r = 0.95; y = O.95x 0.14; sta...

Stable tethering of bioactive peptides like RGD to surfaces can be achieved via chemical bonding, biotin streptavidin interaction, or photocross-linking. More challenging is the immobilization of proteins, since methods applied to immobilize peptides are either not specific or versatile enough or might even compromise the protein's bioactivity. To overcome this limitation, we have employed a sc...

The ability to predictably control the coassembly of multiple nanoscale building blocks, especially those with disparate chemical and physical properties such as biomolecules and inorganic nanoparticles, has far-reaching implications in catalysis, sensing, and photonics, but a generalizable strategy for engineering specific contacts between these particles is an outstanding challenge. This is e...

the present study was aimed at the development of a somoclonal variant and isozyme marker for phyllanthus amarus schum & thonn using inter-nodal segments and the enzyme peroxidase. maximum callus proliferation was obtained on murashige and skoog’s medium supplemented (ms) with 1.0 mg/l of 2,4-dichlorophenoxyacetic acid. three weeks-old pale yellowish white semi-friable callus was used for o...

Extraction of steroids from urine with C18 solid-phase extraction cartridges results in an extract containing impurities. If, during the extraction of hydrolyzed urine, an amino (NH2) column is placed in series with the C18 column, then one obtains a sample that is sufficiently clean for gas-chromatographic analysis. Analytical recovery of dehydroepiandrosterone from urine is considerably decre...

In this paper we consider dipole-mediated correlations between DNA and enzymes in the context of their water environment. Such correlations emerge from electric dipole-dipole interactions between aromatic ring structures in DNA and in enzymes. We show that there are matching collective modes between DNA and enzyme dipole fields, and that a dynamic time-averaged polarization vanishes in the wate...

Reaction intermediates define the chemical steps of transformation from substrates to products, and capture of intermediates along catalytic pathways remains one focal point in the studies of enzymatic mechanisms. Performing enzymatic reactions in protein single crystals has been shown to be very effective in trapping and identifying unstable intermediates. In this critical review, we provide s...

this study looks at the communication between users concerning health risks, with the aim of exploring their use of fora and assessing whether participants establish a niche with like-minded users during these exchanges. by integrating a corpus linguistic approach with content analysis and multiple studies on computer mediated health discourse, this study analyses the intense attention paid to ...

BEFORE the introduction of enzymatic zonulysis by Joaquin Barraquer (1958), the normal procedure of senile lens extraction was either by intraor extra-capsular extraction, or, more recently, by the use of the erisophake. This latter operation can be done with much greater facility by the use of the enzyme, as has been demonstrated by Barraquer (1958), Cogan (1958), and Cogan, Symons, and Gibbs ...