fast enzyme linked immunosorbent assay (fast-elisa) was compared with the standard elisa for the diagnosis of human hydatidosis. seventy serum samples including 30 from hydatidosis patients (surgically confirmed), healthy control individuals not infected with any parasitic diseases (n=/20) and from others with different parasitic infections including, toxocariosis (n=5), fasciolosis (n=5), tric...

Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that PCR tests, which considered gold standard. In present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve LOD antigen coronavirus disease 2019 (COVID-19). For recombinant...

We have devloped an enzyme-linked immunosorbent assay for determining choriomammotropin (human placental lactogen) in serum. Unlabeled hormone competes with choriomammotropin-beta-galactosidase conjugate for antibody bound to polystyrene tubes. The entire assay can be performed in 2.5 h with good precision. The coefficient of variation for one sample with a mean concentration of 5.6 mg/L, assay...

Modification with gold black considerably increased the detection current of protruding micropillar electrodes in microfluidic electrochemical enzyme-linked immunosorbent assay (ELISA).

To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were col...

there are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals edcs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. the yolk protein precursor vitellogenin (vtg) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to edcs especially...

As the gold standard of protein detection, enzyme-linked immunosorbent assay (ELISA) is widely used in medical treatment and biology. Here, we report a digital ELISA method that combines fluorescence-coded magnetic beads with micropore arrays to effectively improve accuracy detection. Fluorescence coded were as solid support ELISA, which modified specifically capture IL-6 serum, then combined g...

Bullous pemphigoid (BP) is the most common autoimmune bullous disease.1 The association between BP and psoriasis has been frequently reported.2 histopathologic serologic findings in patients with include a subepidermal blister eosinophils3,4 BP180-noncollagenous 16A (NC16A) reactivity, demonstrated using enzyme-linked immunosorbent assay (ELISA).1 Uncommonly, histopathology of may be rich neutr...

This study aimed to evaluate the effect of Wogonoside (Wog), a flavonoid monomer, on hyperlipidemia and explore its possible mechanisms. APOE -/- mice were used establish animal model by feeding high-fat diet (HFD). The serum level triglycerides (TG), total cholesterol (TC), high-density lipoprotein (HDL-c), low-density (LDL-c), inflammatory cytokines measured enzyme-linked immunosorbent assay ...