Gene set enrichment analysis is a microarray data analysis method that uses predefined gene sets and ranks of genes to identify significant biological changes in microarray data sets. In this paper we present a novel method integrating gene interaction information with Gene Ontology (GO) for the construction of new interesting enriched gene sets. The experimental results show that the introduce...

Gene targeting is a powerful method for introducing mutations into the genome of embryonic stem cells. The most widely used approach is the positive-negative selection method in which a gene encoding a negative selection marker is cloned into the replacement vector to obtain an enrichment of properly targeted clones. Here, we present an alternative means to introduce any given negative selectio...

The immunomagnetic separation (IMS) is a technique that has been used to increase sensitivity and specificity and to decrease the time required for detection of Salmonella in foods through different methodologies. In this work we report on the development of a method for detection of Salmonella in chicken cuts using in house antibody-sensitized microspheres associated to conventional plating in...

G. Siwiec, B. Oleksiak, J. Wieczorek Silesian University of Technology, Katowice, Poland A. Blacha-Grzechnik Silesian University of Technology, Gliwice, Poland In the presented study the flotation process has been proposed as a method of enrichment of silver-bearing jewellery waste. This method, traditionally used for the enrichment of non-ferrous metal ores, is based on differences in wettabil...

in order to evaluate the effect of substrate and enrichment material on biological efficiency (be) and other physical and chemical propertiese of medicinal mushroom shiitake (lentinula edodes) an investigation was perfomed as factorial experiment in completely randomized designe with four replications in khorramabad city of lorestan continent iran. experimental factors were substrate in three l...

iron deficiency anemia is one of the nutritional problems of the low income, undernourished populations of the world. in iran, many rural and low socioeconomic urban groups suffer from iron deficiency anemia because of a low iron intake and the low level of public health. to overcome and to prevent iron deficiency, a program was suggested to enrich the bread with iron, calcium and some of the b...

A methodology to model arbitrary holes and material interfaces (inclusions) without meshing the internal boundaries is proposed. The numerical method couples the level set method (Osher and Sethian, 1988) to the eXtended Finite Element Method (X-FEM) (Mo es et al., 1999). In the X-FEM, the nite element approximation is enriched by additional functions through the notion of partition of unity. T...

introduction: the suspected water samples contaminated with legionella were enriched with acanthamoebae. small numbers of bacteria (not detectable by culture) can be multiplied in amoebae up to culturable level in co-culture condition. the conventional culture method for legionnaires' disease bacterium always suffers from low sensitivity. this co-culture method can significantly improve culture...

objective(s):  in our study, graphene oxide-tio2 nanocomposite (go/tio2) was prepared and used for the enrichment of rutin from real samples for the first time.  materials and methods:   the synthesized go/tio2 was characterized by x-ray diffraction, scanning electron microscopy, and ft-ir spectra.  the enrichment process is fast and highly efficient. the factors including contact time, ph, and...