AIMS/INTRODUCTION The distributer of the anti-glutamic acid decarboxylase antibody assay kit using radioimmunoassay (RIA) recently announced its discontinuation, and proposed an alternative kit using enzyme-linked immunosorbent assay (ELISA). The aim of the present study was to investigate the diagnostic values of the anti-glutamic acid decarboxylase antibody by RIA and ELISA among type 1 diabe...

Labeling of food allergens in accordance with legal regulations is important to protect the health allergic consumers. The requirements for detecting foods involve adequate specificity and sensitivity identify very small amounts target complex matrices processed foods. In this work, one hundred commercial samples were analyzed walnut detection using three different methods: a sandwich enzyme-li...

Progranulin (PGRN) levels in blood and cerebrospinal fluid (CSF) are increasingly studied as potential markers for neurodegenerative disorders. We aimed to 1) characterize two commercially available PGRN ELISAs on several assay validation parameters, 2) assess the stability of PGRN in serum and CSF under pre-analytical conditions, and 3) compare stability in the two assays. Intra- and inter-ass...

Introduction. Anemia is a frequent problem in hospitalized geriatric patients, and the anemia of chronic disease (ACD) and iron deficiency anemia (IDA) are the 2 most prevalent causes. The aim of the study was to assess the possible role of serum hepcidin in the differential diagnosis between ACD and IDA. Methods. We investigated serum hepcidin, iron status, anemia, and C-reactive protein in 39...

Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAb...

Systemic lupus erythematosus (SLE) is an autoimmune disease and a chronic systematic disease with unknown etiology influential more than one million individuals each year. The aim of this study is to substantiation the impact of IL4, C3 and C4 in patients with Systemic Lupus Erythematosus. During the period 2/January/2013 to 1/October/2013, fifty patients with Systemic Lupus Erythematosus that ...

Rapid detection of soluble F1 capsular antigen in serum, bubo fluid or urine of patients proved to be a valuable tool in the presumptive diagnosis of plague. We evaluated a F1 capsular antigen capture ELISA resembling a commercially available test kit. The minimal detectable concentration was 4 ng/ml. The specificity was 100% when investigating 47 sera from healthy Malagasy subjects and 98.4% w...

A seroprevalence survey of toxoplasmosis among 91 Thai Buddhist monks was conducted in two temples in Nakhon Pathom Province, Thailand. A high prevalence (8.8%) of toxoplasmosis among these subjects was observed. Control of cats and dogs in Buddhist temples should be made a current public health strategy. A total of 91 male Buddhist monks from the two temples were included into this study. Afte...

conclusions the newly developed simple indirect elisa showed high sensitivity and specificity with respect to vnt. developing such a simple, sensitive, and specific elisa which is much less expensive than the available commercial elisa kits can improve the detection of bvdv infections, help to eliminate the disease from herds, and decrease economic losses caused by this disease. background bovi...