Urine is evaluated by electrophoretic methods both to gather information about the location and degree of damage within the nephron and to detect and quantify monoclonal free light chains (Bence Jones proteins). For many years, urine protein electrophoresis consisted of concentrating the urine (usually 50-fold) followed by manual application of the concentrated sample to a gel. Recently, semiau...

We compared the automated Paragon 2000 clinical capillary zone electrophoresis (CZE) system with two manual methods, agarose electrophoresis (AGE) and cellulose acetate electrophoresis (CAE). Reference intervals in healthy adults were determined for each method. When compared with AGE and CAE, CZE gave substantially higher reference values for the alpha1-globulin fraction. With CZE, within-run ...

Electrophoresis is an indispensable technique for research such as separation analysis. A Monoclonal-like peak was discovered in alfa2 region on routine-based capillary zone electrophoresis (CZE) but neither cellulose acetate membrane nor immunofixation. From clinical records it strongly indicated the involvement of iopamidol, radio-contrast medium, administered at CT scan examination. Very hig...

Hairpin pyrrole-imidazole polyamides (hPIPs) and their chlorambucil (Chb) conjugates (hPIP-Chbs) can alkylate DNA in a sequence-specific manner, have been studied as anticancer drugs. Here, we conjugated Chb to cyclic PIP (cPIP), which is known higher binding affinity than the corresponding hPIP, investigated alkylation properties of resulting cPIP-Chb using optimized capillary electrophoresis ...

in this study the effects of water salinity on serum total protein and its components in grass carp were investigated. the aim of this study was to determine the effect of salinity tolerance of fish on total serum protein level and its components as an indicator of liver and kidney activity. one hundred and twenty grass carp were divided into four groups, randomly. the first three groups were r...

egfr is a key molecule in cancer cells. egfr signaling was shown to promote tumor cell proliferation and survival, invasion and angiogenesis and mediate resistance to treatment, including ionizing radiation in preclinical models. we extracted proteins from astrocytoma (iii and iv) oligodendroglioma(iv) tumors and normal brain tissues and then evaluated the protein purity by bradford test and sp...

the phenomenological model of khossravi and connors (1992) has been adopted to calculate the electrophoretic mobility of drugs at different concentrations of solvents in a binary mixture. the accuracy and predictability of the model have been evaluated employing 14 experimental data sets by using average percentage mean deviation (apmd). the obtained apmd for correlative and predictive studies ...

background: the aim of the present study was to induce salivation in haemaphysalis longicornis to increase saliva production and to characterize the collection of proteins present in the collected saliva using on-chip-electrophoresis. methods: saliva of adult female engorged h. longicornis was collected by treatment with 0.2% dopamine hydrochlo­ride. all protein samples were characterized by sd...