A separation-free electrochemical immunoassay method for the detection of the pesticide atrazine is described. The method developed is a competitive ELISA incorporating disposable screen printed horseradish peroxidase modified electrodes as the detector element in conjunction with single-use atrazine immuno-membranes. Screen printed carbon electrodes were prepared using carbon ink incorporating...

We report a novel method of electrochemical signaling from antigen-antibody interactions at immunoelectrodes with bioelectrocatalyzed enzymatic signal amplification. For the immunosensing surface construction, a poly(amidoamine) G4-dendrimer was employed not only as a building block for the electrode surface modification but also as a matrix for ligand functionalization. As a model biorecogniti...

A novel electrochemical immunosensor for the detection of important marine biotoxins domoic acid (DA) and okadaic (OA) was developed. The sensors used carbon black modified screen-printed electrodes (CB-SPE) obtained using a high-throughput method. performance stability CB SPEs bare (c-SPEs) were compared cyclic voltammetry impedance spectroscopy. CB-SPEs showed improved long-term (at least six...

In this work, a sensitive dual mode competitive electrochemical immunosensor based on differential pulse voltammetry (DPV) and square wave (SWV) response modes for quantitative determination of dibutyl phthalate (DBP) was successfully established. According to diethylenetriamine (DETA) assisted hydrothermal strategy, novel N-doped graphene/CoSe2 nanobelt composite prepared. Afterwards, PEI func...

BACKGROUND A reagentless sensor array for simultaneous multianalyte testing (SMAT) may enable accurate diagnosis and be applicable for point-of-care testing. We developed a disposable reagentless immunosensor array for simple immunoassay of panels of tumor markers. METHODS We carried out SMAT with a direct capture format, in which colloidal gold nanoparticles with bound horseradish peroxidase...