An impedimetric biosensor platform for bio-affinity assays was developed based on real-time, label-free electrochemical detection performed via direct interface to electronic digital data processing. The sensor array consists of 15 gold microelectrode pairs enclosed in three reaction chambers and bio-functionalized with specific DNA probes. The impedance change caused by specific target analyte...

Tetrahydrobiopterin (BH4) is an essential co-factor in the biosynthesis of monoamine neurotransmitters. A nano-Pt/Pd modified electrode as the electrochemical detector (ED) for high-performance liquid chromatography (HPLC) coupled with microdialysis sampling, is used to explore the effect of administration of BH4 on the monoamine neurotransmitters in the rat striatum. The researches demonstrate...

A rapid and sensitive assay for quantification of nalbuphine, butorphanol and morphine in blood (50μl) and brain microdialysate (~40μl) samples was developed. Blood samples were extracted with ethyl acetate. Analysis was performed with high performance liquid chromatography coupled to an electrochemical detector. The mobile phase was a mixture of 0.1M sodium phosphate buffer, methanol and octan...

This report describes a versatile and robust microreactor for bioactive proteins physically immobilized on a polyether sulfone filter. The potential of the reactor is illustrated with glucose oxidase immobilized on a filter with a cut-off value of 30 kDa. A flow-injection system was used to deliver the reactants and the device was linked on-line to an electrochemical detector. The microreactor ...

Quaternary-alloyed ZnxCd1-xSySe1-y arrays were prepared from four precursor aqueous/glycerol (3:1) solutions with a robotic piezoelectric dispenser on fluorine-doped tin oxide (FTO) conductive glass. Hydrazine (N2H4) was used for protecting the selenium source, dimethylselenourea, from oxidation so that fabrication of the arrays could be carried out under ambient air conditions. The absorption ...

where TR (v) is the radiation temperature introduced by Planck2 in order to describe the nonequilibrium radiaHon, and T is the working system temperature. The kinetic arrangements for free energy storage are completely ignored in (1). The simplest known photosynthetic mechanism developed by nature is based on the action of protein bacteriorhodopsin, which was isolated from purple membrane patch...

A potentiometric enzyme biosensor is a convenient detector for quantification of urea concentrations in industrial processes, or for monitoring patients with diabetes, kidney damage or liver malfunction. In this work, poly(3-hexylthiophene-co-3-thiopheneacetic acid) (P(3HT-co-3TAA)) was chemically synthesized, characterized and spin-coated onto conductive indium tin oxide (ITO) glass electrodes...

The impurity analysis of ethylene glycol in hydroxyethyl starch (HES) was evaluated using the exact method and conditions described in the official EP 8.2 (2014) monograph [2]. The evaluation was performed using an ALEXYS® LC-EC analyzer for ethylene glycol, based on the DECADE Elite electrochemical detector and a SenCell with Au working electrode (WE) and HyREF (Pd/H 2 ) reference electrode. D...

We describe the use of electrogenerated reactants for continuous on-line reaction detection with thin-layer hydrodynamic amperometry. The reagent is introduced into the liquid-chromatographic column effluent at a constant rate by using controlled-current electrochemistry. After the effluent passes through a short reaction coil, the reagent concentration is monitored at the detector. Reaction of...

The fabrication and properties of n-ZnO nanowires/p-CuO coaxial heterojunction (CH) with a photoresist (PR) blocking layer are reported. In our study, c-plane wurtzite ZnO nanowires were grown by aqueous chemical method, and monoclinic CuO (111) was then coated on the ZnO nanowires by electrochemical deposition to form CH. To improve the device performance, a PR layer was inserted between the Z...