A monoclonal antibody enzyme-linked immunosorbent assay (ELISA) was developed to detect Cryptosporidium parvum oocysts in bovine feces. Fecal oocysts were concentrated by centrifugation through Formalin-ethyl acetate solution and captured with monoclonal antibody 18.280.2 reactive with C. parvum oocysts. Captured oocysts were detected with goat anti-oocyst serum, following the addition of a per...

Between September and November 1991, 12 Owen Stanley skinks, Papuascincus stanleyanus (Booulenger) were collected from various localities on Papua New Guinea and examined for coccidians. Six (50%) were found to harbour four eimerians that we describe here as new. Oocysts of Eimeria burseyi sp. n. were elongate to ellipsoidal with a bilayered wall and measured (length x width, L x W) 36.0 x 24.0...

BACKGROUND Coccidiosis is a major global veterinary health problem in intensively reared chickens. It is caused by apicomplexan parasites of the genus Eimeria. PRINCIPAL FINDINGS A subunit vaccine composed of purified antigens from the gametocytes of Eimeria maxima was used to stimulate the production and transfer of maternal antibodies between breeding hens and their hatchlings. The vaccine ...

Eimeria tenella is one of the important species as it causes caecal coccidiosis in chickens. Coccidiosis is believed to be controlled effectively using vaccines. The present study investigated the prophylactic efficacy of two locally prepared vaccines against caecal coccidiosis in broiler chicken. Broiler chicks (n = 80) were divided in four different groups A, B, C and D (n = 20 birds/group), ...

Ninety six 3-day-old Bowans White strain chicks were divided into a control and 7 experimental groups. Group 1 (control) received normal drinking water for 7 d. Chicks from groups 2, 3 and 4 received 50 ppm of toltrazuril at the begining and on days 3 and 6 of the experiment. Groups 5, 6, 7 and 8 were infected with a single dose of Eimeria tenella oocysts and then groups 6, 7 and 8 received add...

Eimeria spp. are a highly successful group of intracellular protozoan parasites that develop within intestinal epithelial cells of poultry, causing coccidiosis. As a result of resistance against anticoccidial drugs and the expense of manufacturing live vaccines, it is necessary to understand the relationship between Eimeria and its host more deeply, with a view to developing recombinant vaccine...

The effect of green tea extracts on the sporulation of oocysts of 3 species of Eimeria was investigated. The unsporulated oocysts were exposed to 0%, 5% and 10% of green tea extracts (1 and 2% strengths, w/v). A new sporulation inhibition bioassay was designed to evaluate the anticocccidial activity of the tea extracts. The results showed for the first time that water-soluble extracts from gree...

In a previous study, we have developed several chicken monoclonal antibodies (mAbs) against Eimeria acervulina (EA) in order to identify potential ligand molecules of Eimeria. One of these mAbs, 6D-12-G10, was found to recognize a conoid antigen of EA sporozoites and significantly inhibited the sporozoite invasions of host T lymphocytes in vitro. Furthermore, some of these chicken mAbs showed c...

By means of turkeys’ faecal samples flotation with NaCl solution, the species composition of Eimeria spp. has been determined in 16 settlements in the Republic of Bulgaria and 4 settlements in the Republic of Turkey. In Bulgaria, the following species were detected: Eimeria adenoeides; E. meleagridis; E. meleagrimitis; E. gallopavonis; E. subrotunda, as well as Isospora heisini, whereas in Turk...

Abundance of intestinal parasites was monitored by fecal egg and oocyst counts for samples of wild rabbits Oryctolagus cuniculus with different levels of imposed female sterility from 12 populations in southwestern Australia. Differences in egg counts of Trichostrongylus retortaeformis between seasons and age groups were dependent on the sex of the host. Pregnancy may have been responsible for ...